Platelet-Rich Fibrin Accelerates Skin Wound Healing in Diabetic Mice

被引：29

作者：

Ding, Yinjia ^{[1
]}

Cui, Lei ^{[2
]}

Zhao, Qiming ^{[1
]}

Zhang, Weiqiang ^{[1
]}

Sun, Huafeng ^{[1
]}

Zheng, Lijun ^{[1
]}

机构：

[1] PLA Hosp 117, Dept Plast & Reconstruct Surg, 14 Lin Ying Rd, Hangzhou, Zhejiang, Peoples R China

[2] Capital Med Univ, Beijing Shijitan Hosp, Dept Plast Surg, Beijing, Peoples R China

来源：

ANNALS OF PLASTIC SURGERY | 2017年 / 79卷 / 03期

关键词：

platelet-rich fibrin; skin wound healing; diabetic mice; diabetic foot ulcers; CLINICAL-TRIAL; LEUKOCYTE; EVALUATE; PLASMA; ULCERS; GEL;

D O I：

10.1097/SAP.0000000000001091

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Diabetic foot ulcers (DFUs) are associated with an increased risk of secondary infection and amputation. Platelet-rich fibrin (PRF), a platelet and leukocyte concentrate containing several cytokines and growth factors, is known to promote wound healing. However, the effect of PRF on diabetic wound healing has not been adequately investigated. The aim of the study was to investigate the effect of PRF on skin wound healing in a diabetic mouse model. Platelet-rich fibrin was prepared from whole blood of 8 healthy volunteers. Two symmetrical skin wounds per mouse were created on the back of 16 diabetic nude mice. One of the 2 wounds in each mouse was treated with routine dressings (control), whereas the other wound was treated with PRF in addition to routine dressings (test), each for a period of 14 days. Skin wound healing rate was calculated. Use of PRF was associated with significantly improved skin wound healing in diabetic mice. On hematoxylin and eosin and CD31 staining, a significant increase in the number of capillaries and CD31-positive cells was observed, suggesting that PRF may have promoted blood vessel formation in the skin wound. In this study, PRF seemed to accelerate skin wound healing in diabetic mouse models, probably via increased blood vessel formation.

引用

页码：E15 / E19

页数：5

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