Local electrical stimulation of single adherent cells using three-dimensional electrode arrays with small interelectrode distances

被引：8

作者：

Braeken, Dries ^{[1
]}

Huys, Roeland ^{[1
]}

Jans, Danny ^{[1
,2
]}

Loo, Josine ^{[1
]}

Severi, Simone ^{[1
]}

Vleugels, Frank ^{[1
]}

Borghs, Gustaaf ^{[1
]}

Callewaert, Geert ^{[2
]}

Bartic, Carmen ^{[1
]}

机构：

[1] IMEC, Kapeldreef 75, B-3001 Louvain, Belgium

[2] Katholieke Univ Leuven, Neurophysiol Lab, B-8500 Kortrijk, Belgium

来源：

2009 ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-20 | 2009年

基金：

芬兰科学院;

关键词：

MICROELECTRODE ARRAYS; RECORDINGS; MEA;

D O I：

10.1109/IEMBS.2009.5333871

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this paper, we describe the localized and selective electrical stimulation of single cells using a three-dimensional electrode array. The chip consisted of 84 nail-like electrodes with a stimulation surface of 0.8 mu m(2) and interelectrode distances as small as 3 mu m. N2A cells were used to compare bipolar stimulation between one electrode in- and one outside the cell on the one hand, and two electrodes in the same cell on the other hand. Selective and localized stimulation of primary embryonic cardiomyocytes showed the possibility to use this chip with excitable cells. The response of the cells to applied electrical fields was monitored using calcium imaging whereas assessment of electroporation was determined following influx of propidium iodide. Arrays of these three-dimensional electrodes could eventually be used as a tool to selectively electroporate the membrane of single cells for genetic manipulation or to obtain electrical access to the inner compartment of the cell.

引用

页码：2756 / 2759

页数：4

共 12 条

[1] Microfluidics and multielectrode array-compatible organotypic slice culture method [J].