Insights into the evolutionary conserved regulation of Rio ATPase activity

被引:28
作者
Knueppel, Robert [1 ]
Christensen, Regitse H. [2 ,8 ]
Gray, Fiona C. [2 ]
Esser, Dominik [3 ,9 ]
Strauss, Daniela [4 ]
Medenbach, Jan [4 ]
Siebers, Bettina [3 ]
MacNeill, Stuart A. [2 ,5 ]
LaRonde, Nicole [6 ,7 ]
Ferreira-Cerca, Sebastien [1 ]
机构
[1] Univ Regensburg, Inst Biochem Genet & Microbiol, Biochem 3, Univ Str 31, D-93053 Regensburg, Germany
[2] Univ Copenhagen, Copenhagen Bioctr, Dept Biol, Ole Maaloes Vej 5, DK-2200 Copenhagen N, Denmark
[3] Univ Duisburg Essen, Fac Chem, Mol Enzyme Technol & Biochem Biofilm Ctr, Univ Str 5, D-45141 Essen, Germany
[4] Univ Regensburg, Inst Biochem Genet & Microbiol, Biochem 1, Univ Str 31, D-93053 Regensburg, Germany
[5] Univ St Andrews, Sch Biol, St Andrews KY16 9ST, Fife, Scotland
[6] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA
[7] Univ Maryland, Marlene & Stewart Greenebaum Canc Ctr, Baltimore, MD 21201 USA
[8] Rigshosp, TrygFondens Ctr Akt Sundhed, 7641,Blegdamsvej 9, DK-2100 Copenhagen O, Denmark
[9] Boehringer Ingelheim RCV GmbH & Co KG, Dr Boehringer Gasse 5-11, A-1120 Vienna, Austria
基金
美国国家科学基金会;
关键词
PRE-RIBOSOMAL-RNA; ARCHAEON HALOFERAX-VOLCANII; SACCHAROMYCES-CEREVISIAE; CYTOPLASMIC MATURATION; PRE-40S RIBOSOMES; BINDING PROTEINS; IN-VIVO; SUBUNIT; BIOGENESIS; YEAST;
D O I
10.1093/nar/gkx1236
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Eukaryotic ribosome biogenesis is a complex dynamic process which requires the action of numerous ribosome assembly factors. Among them, the eukaryotic Rio protein family members (Rio1, Rio2 and Rio3) belong to an ancient conserved atypical protein kinase/ATPase family required for the maturation of the small ribosomal subunit (SSU). Recent structure-function analyses suggested an ATPase-dependent role of the Rio proteins to regulate their dynamic association with the nascent pre-SSU. However, the evolutionary origin of this feature and the detailed molecular mechanism that allows controlled activation of the catalytic activity remained to be determined. In this work we provide functional evidence showing a conserved role of the archaeal Rio proteins for the synthesis of the SSU in archaea. Moreover, we unravel a conserved RNA-dependent regulation of the Rio ATPases, which in the case of Rio2 involves, at least, helix 30 of the SSU rRNA and the P-loop lysine within the shared RIO domain. Together, our study suggests a ribosomal RNA-mediated regulatory mechanism enabling the appropriate stimulation of Rio2 catalytic activity and subsequent release of Rio2 from the nascent pre-40S particle. Based on our findings we propose a unified release mechanism for the Rio proteins.
引用
收藏
页码:1441 / 1456
页数:16
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