Comparative HPLC-MSn analysis of canine and human meibomian lipidomes: many similarities, a few differences

被引：29
作者：
Butovich, Igor A. [1 ,2 ]
Borowiak, Anna M. [3 ]
Eule, J. Corinna [3 ]
机构：
[1] Univ Texas SW Med Ctr Dallas, Dept Ophthalmol, Dallas, TX 75390 USA
[2] Univ Texas SW Med Ctr Dallas, Grad Sch Biomed Sci, Dallas, TX 75390 USA
[3] Free Univ Berlin, Klin & Poliklin Kleine Haustiere, D-1000 Berlin, Germany
来源：
SCIENTIFIC REPORTS | 2011年 / 1卷
关键词：
CHROMATOGRAPHY-MASS-SPECTROMETRY; FATTY-ACID-COMPOSITION; CORNEAL TEAR FILM; GLAND DYSFUNCTION; CHOLESTERYL ESTERS; OCULAR SURFACE; STEER; RABBIT;
D O I：
10.1038/srep00024
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The aim of this study was to evaluate the lipidome of meibomian gland secretions in canines (cMGS) - a common pet and laboratory animal - and to compare it with that of human MGS (hMGS), to determine whether canines could be used as a valid experimental animal model in studies of the biochemistry and physiology of the human ocular surface in general, and of the Meibomian glands in particular. The MGS of both species were evaluated using HPLC in combination with atmospheric pressure chemical ionization ion trap mass spectrometry. The main lipid classes found in cMGS were very long chain cholesteryl esters, wax esters, (O-acyl)-omega-hydroxy fatty acids (OAHFA), and cholesteryl esters of OAHFA. The lipidomes of cMGS and hMGS were found to be qualitatively similar, which implies similar biosynthetic and biodegradation pathways in canines and humans. However, some quantitative differences between the two were observed.
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页数：12
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