Detection and validation of EST-SSR markers associated with sugar-related traits in sugarcane using linkage and association mapping

被引：47

作者：

Ukoskit, Kittipat ^{[1
]}

Posudsavang, Ganlayarat ^{[1
]}

Pongsiripat, Nattapat ^{[1
]}

Chatwachirawong, Prasert ^{[2
]}

Klomsa-ard, Peeraya ^{[3
]}

Poomipant, Patthinun ^{[4
]}

Tragoonrung, Somvong ^{[5
]}

机构：

[1] Thammasat Univ, Dept Biotechnol, Rangsit Campus, Klongluang 12121, Pathum Thani, Thailand

[2] Kasetsart Univ, Fac Agr, Dept Agron, Kamphaengsean Campus, Nakhon Pathom 73140, Thailand

[3] Mitr Phol Innovat & Res Ctr, 399 Moo 1,Chumphae Phukiao Rd, Phu Khiao 36110, Chaiyaphum, Thailand

[4] Kasetsart Univ, Inst Food Res & Prod Dev, POB 1043, Bangkok 10903, Thailand

[5] Natl Sci & Technol Dev Agcy, Natl Ctr Genet Engn & Biotechnol BIOTEC, 113 Thailand Sci Pk, Khlong Luang 12120, Pathum Thani, Thailand

来源：

GENOMICS | 2019年 / 111卷 / 01期

关键词：

Association mapping; QTL mapping; Sugar-related traits; Sugarcane; SACCHARUM SPP; QTL ANALYSIS; MICROSATELLITE MARKERS; POPULATION-STRUCTURE; GENOME ORGANIZATION; QUANTITATIVE TRAITS; GENETIC DISSECTION; COMPLEX TRAITS; RESISTANCE; YIELD;

D O I：

10.1016/j.ygeno.2018.03.019

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sugar-related traits are of great importance in sugarcane breeding. In the present study, quantitative trait loci (QTL) mapping validated with association mapping was used to identify expressed sequence tag-simple sequence repeats (EST-SSRs) associated with sugar-related traits. For linkage mapping, 524 EST-SSRs, 241 Amplified Fragment Length Polymorphisms, and 10 genomic SSR markers were mapped using 283 F-1 progenies derived from an interspecific cross. Six regions were identified using Multiple QTL Mapping, and 14 unlinked markers using single marker analysis. Association analysis was performed on a set of 200 accessions, based on the mixed linear model. Validation of the EST-SSR markers using association mapping within the target QTL genomic regions identified two EST-SSR markers showing a putative relationship with uridine diphosphate (UDP) glycosyltransferase, and beta-amylase, which are associated with pol and sugar yield. These functional markers can be used for marker-assisted selection of sugarcane.

引用

页码：1 / 9

页数：9

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