Expression of a human polyomavirus oncoprotein and tumour suppressor proteins in medulloblastomas

被引:31
作者
Del Valle, L
Baehring, J
Lorenzana, C
Giordano, A
Khalili, K
Croul, S
机构
[1] Med Coll Penn & Hahnemann Univ, Sch Med, Dept Pathol & Lab Med, Philadelphia, PA 19129 USA
[2] Med Coll Penn & Hahnemann Univ, Sch Med, Dept Neurol, Philadelphia, PA 19102 USA
[3] Temple Univ, Ctr NeuroVirol & Canc Biol, Philadelphia, PA 19122 USA
[4] Thomas Jefferson Univ, Jefferson Med Coll, Dept Pathol Anat & Cell Biol, Philadelphia, PA 19107 USA
来源
JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY | 2001年 / 54卷 / 05期
关键词
medulloblastoma; T antigen; p53; retinoblastoma protein;
D O I
10.1136/mp.54.5.331
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Aims-Although the aetiology of medulloblastoma remains elusive, several lines of evidence suggest an association with the human neurotropic polyomavirus JC and its oncoprotein T antigen. The tumour forming properties of JC virus T antigen are the result, at least in part, of its ability to bind and inactivate tumour suppressor/ cell cycle regulatory proteins, such as p53 and the retinoblastoma family of proteins. Methods-To examine potential relations between these factors, immunohistochemistry was used to determine associations between the T antigen and the expression of p53 and the retinoblastoma proteins pRb, p107, and Rb2/p130 in eight medulloblastomas. Results-Only the three medulloblastomas with T antigen expression also showed nuclear positivity with antibodies to p53. Although immunohistochemistry detected nuclear labelling for pRb in five of the cases, the three that were positive for T antigen showed the highest pRb labelling. The retinoblastoma related proteins p107 and Rb2/p130 were also immunopositive in most T antigen positive medulloblastomas. Double label immunohistochemistry also demonstrated p53 and pRb positivity in the same cells that were T antigen positive. Conclusions-These correlations suggest that associations between T antigen and p53 and/or T antigen and pRb occur in some of these tumours. These data provide indirect evidence that JC virus, acting through T antigen, might be involved in the formation and progression of medulloblastoma.
引用
收藏
页码:331 / 337
页数:7
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