Oxidative stress increases adrenomedullin mRNA levels in cultured rat vascular smooth muscle cells

被引：48
|
作者：
Ando, K
Ito, Y
Kumada, M
Fujita, T
机构：
[1] Univ Tokyo, Sch Med, Dept Internal Med 4, Bunkyo Ku, Tokyo 1128688, Japan
[2] St Lukes Coll Nursing, Tokyo, Japan
来源：
HYPERTENSION RESEARCH-CLINICAL AND EXPERIMENTAL | 1998年 / 21卷 / 03期
关键词：
diethyldithiocarbamate; nitric oxide; superoxide; vascular tone;
D O I：
10.1291/hypres.21.187
中图分类号：
R6 [外科学];
学科分类号：
1002 ; 100210 ;
摘要：
We investigated the effect of oxidative stress on the expression of adrenomedullin (AM) mRNA in cultured rat vascular smooth muscle cells (VSMCs), using diethyldithiocarbamate (DDC), which is known to inhibit endogenous Cu, Zn superoxide dismutase (SOD) and to increase superoxide (O-2(-)). DDC (10 mM) increased O-2(-) levels produced from rat VSMCs in a time-dependent fashion. Concomitantly, DDC increased the expression of AM mRNA for up to 24 h, although it did not affect the expression of beta-actin mRNA. Thus, oxidative stress enhanced AM production in rat VSMCs, which may compensate for oxidative-stress-induced vasoconstriction.
引用
收藏
页码：187 / 191
页数：5
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