Assessment of in vitro interferon-γ responses from peripheral blood mononuclear cells of cattle infected with Mycoplasma mycoides ssp mycoides small colony type

被引：20

作者：

Jores, Joerg ^{[1
]}

Nkando, Isabel ^{[2
]}

Sterner-Kock, Anja ^{[3
]}

Haider, Wolfram ^{[3
]}

Poole, Jane ^{[1
]}

Unger, Hermann ^{[4
]}

Muriuki, Cecilia ^{[1
]}

Wesonga, Hezron ^{[2
]}

Taracha, Evans L. N. ^{[1
]}

机构：

[1] Int Livestock Res Inst, Nairobi 00100, Kenya

[2] Kenyan Agr Res Inst KARI Muguga, Kikuyu, Kenya

[3] Inst Tierpathol, D-13127 Berlin, Germany

[4] IAEA, Joint FAO, Anim Prod & Hlth Sect, Div Nucl Tech Food & Agr, A-1400 Vienna, Austria

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2008年 / 124卷 / 1-2期

基金：

英国惠康基金;

关键词：

Mycoplasma mycoides ssp mycoides small colony; interferon gamma;

D O I：

10.1016/j.vetimm.2008.02.019

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Contagious bovine pleuropneumonia (CBPP) is a lung disease caused by the bacterial pathogen Mycoplasma mycoides ssp. mycoides small colony type (MmmSC). It has been spreading due to a number of factors including poor vaccine efficacy and poor sensitivity of current diagnostic tests. The purpose of this study was to assess interferon gamma (IFN-gamma) release after stimulation of peripheral blood mononuclear cells (PBMC) from experimentally infected cattle. PBMC collected from 15 artificially infected animals were incubated with different concentrations of total MmnzSC antigen. After 72 It of incubation the IFN-gamma release was measured and found to be elevated in I I animals. We did not observe a correlation between IFN-gamma release of animals with and without pathomorphological gross lesions. Therefore, our data do not confirm a role for CD4 T-lymphocytes in protection, since there is no correlation between IFN-g secretion (supposed to be mainly derived from CD4 T-cells) and disease severity. Additionally, we applied immunocytochemistry on affected lung tissue and detected no build up of T-lymphocytes (CD4 T-cells, CD8 T-cells) but a high presence of myeloid cells. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：192 / 197

页数：6

共 11 条

[1]

BERG R, 1990, SPLANCHNOLOGIE, V2

[2] Serodiagnosis and monitoring of contagious bovine pleuropneumonia (CBPP) with an indirect ELISA based on the specific lipoprotein LppQ of Mycoplasma mycoides subsp mycoides SC [J].