Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe

被引：42

作者：

Liu, Gangjun ^{[1
,2
]}

Kieu, Khanh ^{[3
]}

Wise, Frank W. ^{[4
]}

Chen, Zhongping ^{[1
,2
]}

机构：

[1] Univ Calif Irvine, Beckman Laser Inst, Irvine, CA 92612 USA

[2] Univ Calif Irvine, Dept Biomed Engn, Irvine, CA 92697 USA

[3] Univ Arizona, Coll Opt Sci, Tucson, AZ 85721 USA

[4] Cornell Univ, Dept Appl Phys, Ithaca, NY 14853 USA

来源：

JOURNAL OF BIOPHOTONICS | 2011年 / 4卷 / 1-2期

基金：

美国国家卫生研究院;

关键词：

multiphoton processes; nonlinear microscopy; hand-held probe; fiber laser;

D O I：

10.1002/jbio.201000049

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report on the development of a compact multiphoton microscopy (MPM) system that integrates a compact and robust fiber laser with a miniature probe. The all normal dispersion fiber femtosecond laser has a central wavelength of 1.06 mu m, pulse width of 125 fs and average power of more than 1 W. A double cladding photonic crystal fiber was used to deliver the excitation beam and to collect the two-photon signal. The hand-held probe included galvanometer-based mirror scanners, relay lenses and a focusing lens. The packaged probe had a diameter of 16 mm. Second harmonic generation (SHG) images and two-photon excited fluorescence (TPEF) images of biological tissues were demonstrated using the system.

引用

页码：34 / 39

页数：6

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