Monitoring of 3D breast carcinoma cell culture using proton magnetic resonance imaging

被引：3

作者：

Bartusik, Dorota ^{[1
,2
]}

Tomanek, Boguslaw ^{[1
,2
,3
,4
]}

Fallone, Gino ^{[2
,4
]}

机构：

[1] Natl Res Council Canada, Inst Biodiagnost W, Calgary, AB T2N 4N1, Canada

[2] Cross Canc Inst, Dept Med Phys, Edmonton, AB T6G 1Z2, Canada

[3] Polish Acad Sci, Inst Nucl Phys, PL-31342 Krakow, Poland

[4] Univ Alberta, Dept Oncol, Edmonton, AB T6G 1Z2, Canada

来源：

MEDICINAL CHEMISTRY RESEARCH | 2010年 / 19卷 / 09期

关键词：

Breast cancer cells; Glycosaminoglycan; Magnetic resonance imaging; DRUG-DELIVERY; EXCHANGE; PROTEOGLYCANS; INVITRO;

D O I：

10.1007/s00044-009-9259-8

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

We studied two human mammary carcinoma cell lines, MCF-7(NEO-4) HER-2 negative and MCF-7(HER-2) HER-2 positive, in three-dimensional culture. The aim of the study was to determine the glycosaminoglycan (GAG) concentration in breast cancer cell growth ex vivo. We found that GAG concentration produced by both breast cancer cell lines can be measured with high-field magnetic resonance imaging.

引用

页码：1153 / 1161

页数：9

共 26 条

[1] Cell culture: Biology's new dimension [J].

Abbott, A .

NATURE, 2003, 424 (6951) :870-872

[2] COMPARISON OF TRYPAN BLUE-DYE EXCLUSION AND FLUOROMETRIC ASSAYS FOR MAMMALIAN-CELL VIABILITY DETERMINATIONS [J].

ALTMAN, SA ;

RANDERS, L ;

RAO, G .

BIOTECHNOLOGY PROGRESS, 1993, 9 (06) :671-674

[3] T1 AND T2 PROTON NUCLEAR-MAGNETIC-RESONANCE (NMR) RELAXATION-TIMES INVITRO AND HUMAN INTRACRANIAL TUMORS - RESULTS FROM 98 PATIENTS [J].

CHATEL, M ;

DARCEL, F ;

DECERTAINES, J ;

BENOIST, L ;

BERNARD, AM .

JOURNAL OF NEURO-ONCOLOGY, 1986, 3 (04) :315-321

[4] Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures [J].

Debnath, J ;

Muthuswamy, SK ;

Brugge, JS .

METHODS, 2003, 30 (03) :256-268

[5] Proteoglycans: Pericellular and cell surface multireceptors that integrate external stimuli in the mammary gland [J].

Delehedde, M ;

Lyon, M ;

Sergeant, N ;

Rahmoune, H ;

Fernig, DG .

JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA, 2001, 6 (03) :253-273

[6] STUDIES OF GD-DTPA RELAXIVITY AND PROTON-EXCHANGE RATES IN TISSUE [J].

DONAHUE, KM ;

BURSTEIN, D ;

MANNING, WJ ;

GRAY, ML .

MAGNETIC RESONANCE IN MEDICINE, 1994, 32 (01) :66-76

[7] Water diffusion and exchange as they influence contrast enhancement [J].

Donahue, KM ;

Weisskoff, RM ;

Burstein, D .

JMRI-JOURNAL OF MAGNETIC RESONANCE IMAGING, 1997, 7 (01) :102-110

[8]

ESCHENKO TY, 2007, BIOCHEMISTRY MOSC, V72, P1016

[9] Methods in studying ECM degradation [J].

Everts, Vincent ;

Buttle, Dave J. .

METHODS, 2008, 45 (01) :86-92

[10] An extracellular matrix microarray for probing cellular differentiation [J].

Flaim, CJ ;

Chien, S ;

Bhatia, SN .

NATURE METHODS, 2005, 2 (02) :119-125

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