The Maize WRKY Transcription Factor ZmWRKY40 Confers Drought Resistance in Transgenic Arabidopsis

被引：122

作者：

Wang, Chang-Tao ^{[1
]}

Ru, Jing-Na ^{[2
]}

Liu, Yong-Wei ^{[3
]}

Yang, Jun-Feng ^{[4
]}

Li, Meng ^{[1
]}

Xu, Zhao-Shi ^{[2
]}

Fu, Jin-Dong ^{[2
]}

机构：

[1] Beijing Technol & Business Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing Key Lab Plant Resource Res & Dev, Beijing 100048, Peoples R China

[2] Chinese Acad Agr Sci, Inst Crop Sci,Minist Agr, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Biol & Genet Improvement Triticeae Crops, Beijing 100081, Peoples R China

[3] Hebei Acad Agr & Forestry Sci, Inst Genet & Physiol, Plant Genet Engn Ctr Hebei Prov, Shijiazhuang 050051, Hebei, Peoples R China

[4] Hebei Wangfeng Seed Ind Co Ltd, Xingtai 054900, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2018年 / 19卷 / 09期

关键词：

WRKY; abiotic stress; regulatory mechanism; drought tolerance; maize; GENOME-WIDE ANALYSIS; ABIOTIC STRESS TOLERANCE; FACTOR FAMILY; EXPRESSION ANALYSIS; RESPONSIVE GENES; SALT; IDENTIFICATION; DEHYDRATION; SUPERFAMILY; SEQUENCE;

D O I：

10.3390/ijms19092580

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abiotic stresses restrict the growth and yield of crops. Plants have developed a number of regulatory mechanisms to respond to these stresses. WRKY transcription factors (TFs) are plant-specific transcription factors that play essential roles in multiple plant processes, including abiotic stress response. At present, little information regarding drought-related WRKY genes in maize is available. In this study, we identified a WRKY transcription factor gene from maize, named ZmWRKY40. ZmWRKY40 is a member of WRKY group II, localized in the nucleus of mesophyll protoplasts. Several stress-related transcriptional regulatory elements existed in the promoter region of ZmWRKY40. ZmWRKY40 was induced by drought, high salinity, high temperature, and abscisic acid (ABA). ZmWRKY40 could rapidly respond to drought with peak levels (more than 10-fold) at 1 h after treatment. Overexpression of ZmWRKY40 improved drought tolerance in transgenic Arabidopsis by regulating stress-related genes, and the reactive oxygen species (ROS) content in transgenic lines was reduced by enhancing the activities of peroxide dismutase (POD) and catalase (CAT) under drought stress. According to the results, the present study may provide a candidate gene involved in the drought stress response and a theoretical basis to understand the mechanisms of ZmWRKY40 in response to abiotic stresses in maize.

引用

页数：16

共 60 条

[1] The maize WRKY transcription factor ZmWRKY17 negatively regulates salt stress tolerance in transgenic Arabidopsis plants [J].

Cai, Ronghao ;

Dai, Wei ;

Zhang, Congsheng ;

Wang, Yan ;

Wu, Min ;

Zhao, Yang ;

Ma, Qing ;

Xiang, Yan ;

Cheng, Beijiu .

PLANTA, 2017, 246 (06) :1215-1231

[2] Overexpression of a maize WRKY58 gene enhances drought and salt tolerance in transgenic rice [J].

Cai, Ronghao ;

Zhao, Yang ;

Wang, Yufu ;

Lin, Yongxiang ;

Peng, Xiaojian ;

Li, Qian ;

Chang, Yuwei ;

Jiang, Haiyang ;

Xiang, Yan ;

Cheng, Beijiu .

PLANT CELL TISSUE AND ORGAN CULTURE, 2014, 119 (03) :565-577

[3] Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses [J].

Chen, Jiani ;

Nolan, Trevor M. ;

Ye, Huaxun ;

Zhang, Mingcai ;

Tong, Hongning ;

Xin, Peiyong ;

Chu, Jinfang ;

Chu, Chengcai ;

Li, Zhaohu ;

Yin, Yanhai .

PLANT CELL, 2017, 29 (06) :1425-1439

[4] CaWRKY40, a WRKY protein of pepper, plays an important role in the regulation of tolerance to heat stress and resistance to Ralstonia solanacearum infection [J].

Dang, Feng-Feng ;

Wang, Yu-Na ;

Yu, Lu ;

Eulgem, Thomas ;

Lai, Yan ;

Liu, Zhi-Qin ;

Wang, Xu ;

Qiu, Ai-Lian ;

Zhang, Ting-Xiu ;

Lin, Jing ;

Chen, Yan-Sheng ;

Guan, De-Yi ;

Cai, Han-Yang ;

Mou, Shao-Liang ;

He, Shui-Lin .

PLANT CELL AND ENVIRONMENT, 2013, 36 (04) :757-774

[5] Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response [J].

Dong, JX ;

Chen, CH ;

Chen, ZX .

PLANT MOLECULAR BIOLOGY, 2003, 51 (01) :21-37

[6] The WRKY superfamily of plant transcription factors [J].

Eulgem, T ;

Rushton, PJ ;

Robatzek, S ;

Somssich, IE .

TRENDS IN PLANT SCIENCE, 2000, 5 (05) :199-206

[7] A stress-responsive NAC transcription factor SNAC3 confers heat and drought tolerance through modulation of reactive oxygen species in rice [J].

Fang, Yujie ;

Liao, Kaifeng ;

Du, Hao ;

Xu, Yan ;

Song, Huazhi ;

Li, Xianghua ;

Xiong, Lizhong .

JOURNAL OF EXPERIMENTAL BOTANY, 2015, 66 (21) :6803-6817

[8] Advances in Drought Resistance of Rice [J].

Farooq, Muhammad ;

Wahid, Abdul ;

Lee, Dong-Jin ;

Ito, Osamu ;

Siddique, Kadambot H. M. .

CRITICAL REVIEWS IN PLANT SCIENCES, 2009, 28 (04) :199-217

[9] The soybean GmDi19-5 interacts with GmLEA3.1 and increases sensitivity of transgenic plants to abiotic stresses [J].

Feng, Zhi-Juan ;

Cui, Xiao-Yu ;

Cui, Xi-Yan ;

Chen, Ming ;

Yang, Guang-Xiao ;

Ma, You-Zhi ;

He, Guang-Yuan ;

Xu, Zhao-Shi .

FRONTIERS IN PLANT SCIENCE, 2015, 6

[10] Sequence and analysis of chromosome 2 of Dictyostelium discoideum [J].

Glöckner, G ;

Eichinger, L ;

Szafranski, K ;

Pachebat, JA ;

Bankier, AT ;

Dear, PH ;

Lehmann, D ;

Baumgart, C ;

Parra, G ;

Abril, JF ;

Guigó, R ;

Kumpf, K ;

Tunggal, B ;

Cox, E ;

Quail, MA ;

Platzer, M ;

Rosenthal, A ;

Noegel, AA .

NATURE, 2002, 418 (6893) :79-85

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