Molecular cloning and characterization of the global regulator LaeA in Penicillium citrinum

被引：18
作者：
Xing, Wei [1 ]
Deng, Chao [2 ]
Hu, Chang-Hua [1 ,2 ]
机构：
[1] Southwest Univ, Inst Modern Biopharmaceut, Sch Pharmaceut Sci, Chongqing 400716, Peoples R China
[2] Univ Wollongong, Sch Hlth Sci, Wollongong, NSW 2522, Australia
来源：
BIOTECHNOLOGY LETTERS | 2010年 / 32卷 / 11期
关键词：
Cloning; Global regulator LaeA; Mevastatin; Penicillium citrinum; SECONDARY METABOLISM; GENE-CLUSTER; COMPACTIN; BINDING;
D O I：
10.1007/s10529-010-0375-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We have cloned and analysed a laeA gene (Pci-laeA) that may control mevastatin biosynthesis in Penicillium citrinum. The full-length Pci-laeA sequence is 1,340 bp with an ORF of 1,284 bp encoding 427 amino acids. It shows 95% identity with LaeA from P. chrysogenum. The predicted molecular mass of Pci-LaeA is 48.72 kDa with an estimated theoretical isoelectric point of 6.96. Pci-LaeA has a conserved S-adenosylmethionine binding site and a potential MlcR (a pathway specific regulator in mevastatin biosynthesis) binding site.
引用
收藏
页码：1733 / 1737
页数：5
相关论文
共 16 条
  • [1] Molecular cloning and characterization of an ML-236B (compactin) biosynthetic gene cluster in Penicillium citrinum
    Abe, Y
    Suzuki, T
    Ono, C
    Iwamoto, K
    Hosobuchi, M
    Yoshikawa, H
    [J]. MOLECULAR GENETICS AND GENOMICS, 2002, 267 (05) : 636 - 646
  • [2] MlcR, a zinc cluster activator protein, is able to bind to a single (A/T)CGG site of cognate asymmetric motifs in the ML-236B (compactin) biosynthetic gene cluster
    Baba, Satoshi
    Kinoshita, Hiroshi
    Hosobuchi, Masahiko
    Nihira, Takuya
    [J]. MOLECULAR GENETICS AND GENOMICS, 2009, 281 (06) : 627 - 634
  • [3] Genomic mining for Aspergillus natural products
    Bok, JW
    Hoffmeister, D
    Maggio-Hall, LA
    Murillo, R
    Glasner, JD
    Keller, NP
    [J]. CHEMISTRY & BIOLOGY, 2006, 13 (01): : 31 - 37
  • [4] LaeA, a regulator of morphogenetic fungal virulence factors
    Bok, JW
    Balajee, SA
    Marr, KA
    Andes, D
    Nielsen, KF
    Frisvad, JC
    Keller, NP
    [J]. EUKARYOTIC CELL, 2005, 4 (09) : 1574 - 1582
  • [5] LaeA, a regulator of secondary metabolism in Aspergillus spp.
    Bok, JW
    Keller, NP
    [J]. EUKARYOTIC CELL, 2004, 3 (02) : 527 - 535
  • [6] Accurate prediction of the Aspergillus nidulans terrequinone gene cluster boundaries using the transcriptional regulator LaeA
    Bouhired, Sarah
    Weber, Monika
    Kempf-Sontag, Anita
    Keller, Nancy P.
    Hoffmeister, Dirk
    [J]. FUNGAL GENETICS AND BIOLOGY, 2007, 44 (11) : 1134 - 1145
  • [7] COMPETITIVE INHIBITION OF 3-HYDROXY-3-METHYLGLUTARYL COENZYME A REDUCTASE BY ML-236A AND ML-236B FUNGAL METABOLITES, HAVING HYPOCHOLESTEROLEMIC ACTIVITY
    ENDO, A
    KURODA, M
    TANZAWA, K
    [J]. FEBS LETTERS, 1976, 72 (02) : 323 - 326
  • [8] Sequence-specific binding by Aspergillus nidulans AflR, a C6 zinc cluster protein regulating mycotoxin biosynthesis
    Fernandes, M
    Keller, NP
    Adams, TH
    [J]. MOLECULAR MICROBIOLOGY, 1998, 28 (06) : 1355 - 1365
  • [9] Probing the S-adenosylmethionine-binding site of rat guanidinoacetate methyltransferase - Effect of site-directed mutagenesis of residues that are conserved across mammalian non-nucleic acid methyltransferases
    Hamahata, A
    Takata, Y
    Gomi, T
    Fujioka, M
    [J]. BIOCHEMICAL JOURNAL, 1996, 317 : 141 - 145
  • [10] Requirement of LaeA for secondary metabolism and sclerotial production in Aspergillus flavus
    Kale, Shubha P.
    Milde, Lane
    Trapp, Marisa K.
    Frisvad, Jens C.
    Keller, Nancy P.
    Bok, Jin Woo
    [J]. FUNGAL GENETICS AND BIOLOGY, 2008, 45 (10) : 1422 - 1429
12