Enhancement of Antiproliferative and Proapoptotic Effects of Cadmium Chloride Combined with hSmac in Hepatocellular Carcinoma Cells

被引：8

作者：

Guo, Caixia ^{[1
]}

Li, Yanbo ^{[1
]}

Zhang, Haixia ^{[2
,3
]}

Wang, Zhicheng ^{[3
]}

Jin, Minghua ^{[3
]}

Zhang, Long ^{[3
]}

An, Liping ^{[3
]}

Hu, Guiqin ^{[3
]}

Liu, Xiaomei ^{[3
]}

Liu, Ying ^{[3
]}

Du, Haiying ^{[3
]}

Sun, Zhiwei ^{[1
,3
]}

机构：

[1] Capital Med Univ, Sch Publ Hlth & Family Med, Beijing, Peoples R China

[2] Chaoyang Dist Ctr Dis Control & Prevent, Beijing, Peoples R China

[3] Jilin Univ, Sch Publ Hlth, Changchun 130023, Peoples R China

来源：

CHEMOTHERAPY | 2011年 / 57卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Tumor cells; Cytotoxicity; In vitro; Apoptosis; Combination chemotherapy; Chemosensitivity; CYTOCHROME-C; SMAC/DIABLO RELEASE; INDUCED INHIBITION; BREAST-CANCER; TUMOR-CELLS; APOPTOSIS; MITOCHONDRIA; EXPRESSION; SMAC; LUNG;

D O I：

10.1159/000321031

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: To study the effects of cadmium chloride (CdCl2) combined with hSnnac on the proliferation and apoptosis of hepatocellular carcinoma cells, i.e. SMMC-7721. Methods:SMMC-7721 cells were transfected with pcDNA3.1(+)-hSmac using a lipofectamine-mediated method, and then cell viability was detected by MTT assay after exposure to 10, 20, and 30 mu mol/l CdCl2. Apoptosis was determined by both acridine orange-ethidium bromide staining and flow cytometry, and expressions of caspase-3, caspase-9, and cytochrome c by Western blot. Results: CdCl2 had cytotoxicity to SMMC-7721 cells, and it could inhibit proliferation in a dose-dependent manner and induce apoptosis; hSmac could inhibit proliferation and induce apoptosis independently in SMMC-7721 cells. Furthermore, cotreatment with CdCl2 and hSmac could enhance antiproliferative and proapoptotic effects in SMMC-7721 cells. Conclusions: hSmac could enhance the cytotoxicity of CdCl2. Copyright (C) 2011 S. Karger AG, Basel

引用

页码：27 / 34

页数：8

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