Identification of a polymorphic Plasmodium vivax microsatellite marker

被引：42

作者：

Gomez, JC

McNamara, DT

Bockarie, MJ

Baird, JK

Carlton, JM

Zimmerman, PA ^{[1
]}

机构：

[1] Case Western Reserve Univ, Dept Pathol, Cleveland, OH 44106 USA

[2] Case Western Reserve Univ, Sch Med, Ctr Global Hlth & Dis, Cleveland, OH 44106 USA

[3] Papua New Guinea Inst Med Res, Madang, Papua N Guinea

[4] Amer Embassy Jakarta, USN Med Res Unit 2, Parasit Dis Program, FPO, AP 96520 USA

[5] Inst Genom Res, Parasite Genom Grp, Rockville, MD 20850 USA

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2003年 / 69卷 / 04期

关键词：

D O I：

10.4269/ajtmh.2003.69.377

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Microsatellite markers derived from simple sequence repeats have been useful in studying a number of human pathogens, including the human malaria parasite Plasmodium falciparum. Genetic markers for P. vivax would likewise help elucidate the genetics and population characteristics of this other important human malaria parasite. We have identified a locus in a P. vivax telomeric clone that contains simple sequence repeats. Primers were designed to amplify this region using a two-step semi-nested polymerase chain reaction protocol. The primers did not amplify template obtained from non-infected individuals, nor DNA from primates infected with the other human malaria parasites (P. ovale, P. malariae, or P. falciparum). The marker was polymorphic in P. vivax-infected field isolates obtained from Papua New Guinea, Indonesia and Guyana. This microsatellite marker may be useful in genetic and epidemiologic studies of P. vivax malaria.

引用

页码：377 / 379

页数：3

共 19 条

[11] Genome sequence of the human malaria parasite Plasmodium falciparum [J].

Gardner, MJ ;

Hall, N ;

Fung, E ;

White, O ;

Berriman, M ;

Hyman, RW ;

Carlton, JM ;

Pain, A ;

Nelson, KE ;

Bowman, S ;

Paulsen, IT ;

James, K ;

Eisen, JA ;

Rutherford, K ;

Salzberg, SL ;

Craig, A ;

Kyes, S ;

Chan, MS ;

Nene, V ;

Shallom, SJ ;

Suh, B ;

Peterson, J ;

Angiuoli, S ;

Pertea, M ;

Allen, J ;

Selengut, J ;

Haft, D ;

Mather, MW ;

Vaidya, AB ;

Martin, DMA ;

Fairlamb, AH ;

Fraunholz, MJ ;

Roos, DS ;

Ralph, SA ;

McFadden, GI ;

Cummings, LM ;

Subramanian, GM ;

Mungall, C ;

Venter, JC ;

Carucci, DJ ;

Hoffman, SL ;

Newbold, C ;

Davis, RW ;

Fraser, CM ;

Barrell, B .

NATURE, 2002, 419 (6906) :498-511

[12] Malaria infections are randomly distributed in diverse holoendemic areas of Papua New Guinea [J].

Mehlotra, RK ;

Kasehagen, LJ ;

Baisor, M ;

Lorry, K ;

Kazura, JW ;

Bockarie, MJ ;

Zimmerman, PA .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2002, 67 (06) :555-562

[13] The neglected burden of Plasmodium vivax malaria [J].

Mendis, K ;

Sina, BJ ;

Marchesini, P ;

Carter, R .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2001, 64 (1-2) :97-106

[14]

Nei M, 2000, MOL EVOLUTIONARY PHY

[15] A genetic map and recombination parameters of the human malaria parasite Plasmodium falciparum [J].

Su, XZ ;

Ferdig, MT ;

Huang, YM ;

Huynh, CQ ;

Liu, A ;

You, JT ;

Wootton, JC ;

Wellems, TE .

SCIENCE, 1999, 286 (5443) :1351-1353

[16] Complex polymorphisms in an similar to 330 kDa protein are linked to chloroquine-resistant P-falciparum in Southeast Asia and Africa [J].

Su, XZ ;

Kirkman, LA ;

Fujioka, H ;

Wellems, TE .

CELL, 1997, 91 (05) :593-603

[17] Very high risk of therapeutic failure with chloroquine for uncomplicated Plasmodium falciparum and P-vivax malaria in Indonesian Papua [J].

Sumawinata, IW ;

Leksana, B ;

Sutamihardja, A ;

Purnomo ;

Subianto, B ;

Sekartuti ;

Fryauff, DJ ;

Baird, JK .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2003, 68 (04) :416-420

[18] The sequence of a 200 kb portion of a Plasmodium vivax chromosome reveals a high degree of conservation with Plasmodium falciparum chromosome 3 [J].

Tchavtchitch, M ;

Fischer, K ;

Huestis, R ;

Saul, A .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2001, 118 (02) :211-222

[19] Genetic diversity and chloroquine selective sweeps in Plasmodium falciparum [J].

Wootton, JC ;

Feng, XR ;

Ferdig, MT ;

Cooper, RA ;

Mu, JB ;

Baruch, DI ;

Magill, AJ ;

Su, XZ .

NATURE, 2002, 418 (6895) :320-323

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