Identification of a polymorphic Plasmodium vivax microsatellite marker

被引：42
作者：
Gomez, JC
McNamara, DT
Bockarie, MJ
Baird, JK
Carlton, JM
Zimmerman, PA [1 ]
机构：
[1] Case Western Reserve Univ, Dept Pathol, Cleveland, OH 44106 USA
[2] Case Western Reserve Univ, Sch Med, Ctr Global Hlth & Dis, Cleveland, OH 44106 USA
[3] Papua New Guinea Inst Med Res, Madang, Papua N Guinea
[4] Amer Embassy Jakarta, USN Med Res Unit 2, Parasit Dis Program, FPO, AP 96520 USA
[5] Inst Genom Res, Parasite Genom Grp, Rockville, MD 20850 USA
来源：
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2003年 / 69卷 / 04期
关键词：
D O I：
10.4269/ajtmh.2003.69.377
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
Microsatellite markers derived from simple sequence repeats have been useful in studying a number of human pathogens, including the human malaria parasite Plasmodium falciparum. Genetic markers for P. vivax would likewise help elucidate the genetics and population characteristics of this other important human malaria parasite. We have identified a locus in a P. vivax telomeric clone that contains simple sequence repeats. Primers were designed to amplify this region using a two-step semi-nested polymerase chain reaction protocol. The primers did not amplify template obtained from non-infected individuals, nor DNA from primates infected with the other human malaria parasites (P. ovale, P. malariae, or P. falciparum). The marker was polymorphic in P. vivax-infected field isolates obtained from Papua New Guinea, Indonesia and Guyana. This microsatellite marker may be useful in genetic and epidemiologic studies of P. vivax malaria.
引用
收藏
页码：377 / 379
页数：3
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