The Effect of Silver Nanoparticles on Antioxidant/Pro-Oxidant Balance in a Murine Model

被引：77

作者：

Docea, Anca Oana ^{[1
]}

Calina, Daniela ^{[2
]}

Buga, Ana Maria ^{[3
]}

Zlatian, Ovidiu ^{[4
]}

Paoliello, M. M. B. ^{[5
,6
]}

Mogosanu, George Dan ^{[7
]}

Streba, Costin Teodor ^{[8
]}

Popescu, Elena Leocadia ^{[9
]}

Stoica, Alexandra Elena ^{[10
]}

Birca, Alexandra Catalina ^{[10
]}

Vasile, Bogdan Stefan ^{[10
]}

Grumezescu, Alexandru Mihai ^{[10
]}

Mogoanta, Laurentiu ^{[11
]}

机构：

[1] Univ Med & Pharm Craiova, Dept Toxicol, Craiova 200349, Romania

[2] Univ Med & Pharm Craiova, Dept Clin Pharm, Craiova 200349, Romania

[3] Univ Med & Pharm Craiova, Dept Biochem, Craiova 200349, Romania

[4] Univ Med & Pharm Craiova, Dept Microbiol, Craiova 200349, Romania

[5] Univ Estadual Londrina, Ctr Hlth Sci, Grad Program Publ Hlth, 60 Robert Koch Ave, BR-86038350 Londrina, Parana, Brazil

[6] Albert Einstein Coll Med, Dept Mol Pharmacol, Forchheimer 209,1300 Morris Pk Ave, Bronx, NY 10461 USA

[7] Univ Med & Pharm Craiova, Dept Pharmacognosy & Phytotherapy, Fac Pharm, Craiova 200349, Romania

[8] Univ Med & Pharm Craiova, Dept Res Methodol, Craiova 200349, Romania

[9] Univ Med & Pharm Craiova, Doctoral Sch, Craiova 200349, Romania

[10] Univ Politehn Bucuresti, Fac Appl Chem & Mat Sci, Dept Sci & Engn Oxide Mat & Nanomat, Bucharest 011061, Romania

[11] Univ Med & Pharm Craiova, Dept Histol, Craiova 200349, Romania

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2020年 / 21卷 / 04期

关键词：

silver nanoparticles; oxidative stress; antioxidant activity; subacute toxicity; STRESS-DEPENDENT TOXICITY; OXIDATIVE STRESS; EXPOSURE; CYTOTOXICITY; CHEMICALS; CARRIERS; DRUG; SIZE;

D O I：

10.3390/ijms21041233

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study aimed to evaluate the subacute effect of two types of Ag-NPs(EG-AgNPs and PVP-EG-AgNPs) on antioxidant/pro-oxidant balance in rats. Seventy Wistar rats (35 males and 35 females) were divided in 7 groups and intraperitoneally exposed for 28 days to 0, 1, 2 and 4 mg/kg bw/day EG-Ag-NPs and 1, 2 and 4 mg/kg bw/day PVP- EG-Ag-NPs. After 28 days, the blood was collected, and the total antioxidant capacity (TAC), thiobarbituric reactive species (TBARS),protein carbonyl (PROTC) levels, reduced glutathione (GSH) levels and catalase (CAT) activity were determined. EG-Ag-NPs determined protective antioxidant effects in a dose-dependent manner. The exposure to the 4 mg/kg bw/day EG-Ag-NPs determines both in males and females a significant increase in TAC and CAT and a significant decrease in TBARS and PROTC only in females. The PVP-EG-AgNPs showed a different trend compared to EG-AgNPs. At 4 mg/kg bw/day the PVP-EG-AgNPs induce increased PROTC levels and decreased GSH (males and females) and TAC levels (males). The different mechanisms of EG-AgNPs and PVP-EG-AgNPs on antioxidant-/pro-oxidant balance can be explained by the influence of coating agent used for the preparation of the nanoparticles in the formation and composition of protein corona that influence their pathophysiology in the organism.

引用

页数：17

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