Isolation and Characterization of Exopolysaccharides Produced by the Cyanobacterium Limnothrix redekei PUPCCC 116

被引：45

作者：

Khattar, J. I. S. ^{[1
]}

Singh, D. P. ^{[1
]}

Jindal, Namita ^{[1
]}

Kaur, N. ^{[2
]}

Singh, Y. ^{[1
]}

Rahi, P. ^{[2
]}

Gulati, A. ^{[2
]}

机构：

[1] Punjabi Univ, Dept Bot, Patiala 147002, Punjab, India

[2] Inst Himalayan Bioresource Technol, Palampur 176061, Himachal Prades, India

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2010年 / 162卷 / 05期

关键词：

Cyanobacterium; Exopolysaccharides; Limnothrix redekei; Monomer composition; Rheology; 16S rRNA gene; EXTRACELLULAR POLYMERIC SUBSTANCES; NOSTOC STRAINS; POLYSACCHARIDES; MARINE; REMOVAL;

D O I：

10.1007/s12010-010-8922-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Limnothrix redekei PUPCCC 116, a filamentous cyanobacterium, has been identified through 16S rRNA gene sequencing. Exopolysaccharides (EPS) of this organism have been isolated and characterized chemically, and its rheological properties were compared with commercial xanthan. The organism produced 304 A mu g EPS/ml culture in 21 days. The rate of EPS production was maximum (313 A mu g EPS/mg protein/day) during the initial days of growth, and it decreased to 140 A mu g EPS/mg protein/day during 18-21 days of growth. Chemical analysis of EPS revealed the presence of glucose/mannose, ribose, rhamnose, and uronic acid. Fourier transformed infrared spectrum of EPS further revealed the presence of methyl and carboxyl groups besides C-N groups indicating the presence of peptidyl moieties. Elemental analysis of EPS showed the presence of 4.97% N. The organism under continuous light produced 102% more EPS compared to when grown under a light/dark cycle of 14/10 h. The rheological properties of EPS were comparable with commercial xanthan gum.

引用

页码：1327 / 1338

页数：12

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