Evidence for the plasma membrane localization of Al-activated malate transporter (ALMT1)

被引：73

作者：

Yamaguchi, M ^{[1
]}

Sasaki, T ^{[1
]}

Sivaguru, M ^{[1
]}

Yamamoto, Y ^{[1
]}

Osawa, H ^{[1
]}

Ahn, SJ ^{[1
]}

Matsumoto, H ^{[1
]}

机构：

[1] Okayama Univ, Bioresources Res Inst, Kurashiki, Okayama 7100046, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2005年 / 46卷 / 05期

关键词：

aluminum stress; aluminum-activated malate transporter; green fluorescent protein; plasma membrane; Triticum aestivum L;

D O I：

10.1093/pcp/pci083

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Aluminum (Al)-activated malate transporter (ALMT1) was recently identified from wheat ( Triticum aestivum). Heterologous expression of ALMT1 led to higher malate exudation that is associated with enhanced Al tolerance in transgenic plants. Here, we show the first direct evidence that ALMT1 is localized in the plasma membrane of Al-tolerant wheat. Phase partitioning experiments showed that this transporter was associated with the plasma membrane fraction. ALMT1 was detected in an Al-tolerant wheat line even without Al treatments. Analysis of transient expression of ALMT1:: green fluorescent protein (GFP) in onion and tobacco cells further confirmed this ALMT1 localization.

引用

页码：812 / 816

页数：5

共 13 条

[1] Engineered GFP as a vital reporter in plants [J].

Chiu, WL ;

Niwa, Y ;

Zeng, W ;

Hirano, T ;

Kobayashi, H ;

Sheen, J .

CURRENT BIOLOGY, 1996, 6 (03) :325-330

[2] ALUMINUM TOLERANCE IN WHEAT (TRITICUM-AESTIVUM L) .2. ALUMINUM-STIMULATED EXCRETION OF MALIC-ACID FROM ROOT APICES [J].

DELHAIZE, E ;

RYAN, PR ;

RANDALL, PJ .

PLANT PHYSIOLOGY, 1993, 103 (03) :695-702

[3] Engineering high-level aluminum tolerance in barley with the ALMT1 gene [J].

Delhaize, E ;

Ryan, PR ;

Hebb, DM ;

Yamamoto, Y ;

Sasaki, T ;

Matsumoto, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (42) :15249-15254

[4] A rapid increase in the level of binding protein (BiP) is accompanied by synthesis and degradation of storage proteins in pumpkin cotyledons [J].

Hatano, K ;

Shimada, T ;

Hiraiwa, N ;

Nishimura, M ;

HaraNishimura, I .

PLANT AND CELL PHYSIOLOGY, 1997, 38 (03) :344-351

[5]

Ikegawa H, 2000, SOIL SCI PLANT NUTR, V46, P503

[6]

LARSSON C, 1987, METHOD ENZYMOL, V148, P558

[7] Possible involvement of protein phosphorylation in aluminum-responsive malate efflux from wheat root apex [J].

Osawa, H ;

Matsumoto, H .

PLANT PHYSIOLOGY, 2001, 126 (01) :411-420

[8]

RYAN PR, 1995, PLANTA, V196, P103, DOI 10.1007/BF00193223

[9] A wheat gene encoding an aluminum-activated malate transporter [J].

Sasaki, T ;

Yamamoto, Y ;

Ezaki, B ;

Katsuhara, M ;

Ahn, SJ ;

Ryan, PR ;

Delhaize, E ;

Matsumoto, H .

PLANT JOURNAL, 2004, 37 (05) :645-653

[10] Aluminum rapidly depolymerizes cortical microtubules and depolarizes the plasma membrane: Evidence that these responses are mediated by a glutamate receptor [J].

Sivaguru, M ;

Pike, S ;

Gassmann, W ;

Baskin, TI .

PLANT AND CELL PHYSIOLOGY, 2003, 44 (07) :667-675

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