High-speed counter-current chromatography combined with column chromatography for isolation of methyllycaconitine from Delphinium pseudocyanthum

被引：24

作者：

Gu, DongYu ^{[1
,2
]}

Yang, Yi ^{[1
,2
]}

Zhong, Jie ^{[1
]}

Aisa, Haji Akber ^{[1
]}

Zhang, TianYou ^{[1
,3
]}

机构：

[1] Chinese Acad Sci, Xinjiang Key Lab Plant Resource & Natr Products C, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China

[2] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

[3] Beijing Inst New Technol Applicat, Beijing 100049, Peoples R China

来源：

CHROMATOGRAPHIA | 2007年 / 66卷 / 11-12期

关键词：

high-speed counter-current chromatography; Delphinium pseudocyanthum; methyllycaconitine;

D O I：

10.1365/s10337-007-0432-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Preparative high-speed counter-current chromatography (HSCCC) combined with conventional column chromatography (CC) has been used for isolation and purification of methyllycaconitine from Delphinium pseudocyanthum. n-Hexane-ethyl acetate-methanol-water, 1:1:1:2 (v/v), was used as the solvent system for HSCCC. Separation of methyllycaconitine from an HSCCC fraction was successfully achieved by CC on silica gel using chloroform-methanol, 7:1 (v/v), as mobile phase. A total of 113.45 mg methyllycaconitine of purity > 95% was obtained from 1.044 g extract of D. pseudocyanthum. Its structure was identified by MS and NMR.

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收藏

页码：949 / 951

页数：3

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