Synthetic Analyses of Single-Cell Transcriptomes from Multiple Brain Organoids and Fetal Brain

被引：148

作者：

Tanaka, Yoshiaki ^{[1
]}

Cakir, Bilal ^{[1
]}

Xiang, Yangfei ^{[1
]}

Sullivan, Gareth J. ^{[2
,3
,4
]}

Park, In-Hyun ^{[1
]}

机构：

[1] Yale Sch Med, Dept Genet, Yale Stem Cell Ctr, New Haven, CT 06520 USA

[2] Univ Oslo, Inst Basic Med Sci, Hybrid Technol Hub Ctr Excellence, Oslo, Norway

[3] Oslo Univ Hosp, Dept Pediat Res, POB 1112 Blindern, N-0317 Oslo, Norway

[4] Univ Oslo, POB 1112 Blindern, N-0317 Oslo, Norway

来源：

CELL REPORTS | 2020年 / 30卷 / 06期

关键词：

PLURIPOTENT STEM-CELLS; CEREBRAL ORGANOIDS; HUMAN ES; DIFFERENTIATION; DIVERSITY; MODELS; OLIGODENDROCYTES; INTERNEURONS; FOREBRAIN; DYNAMICS;

D O I：

10.1016/j.celrep.2020.01.038

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Human brain organoid systems offer unprecedented opportunities to investigate both neurodevelopmental and neurological disease. Single-cell-based transcriptomics or epigenomics have dissected the cellular and molecular heterogeneity in the brain organoids, revealing a complex organization. Similar but distinct protocols from different labs have been applied to generate brain organoids, providing a large resource to perform a comparative analysis of brain developmental processes. Here, we take a systematic approach to compare the single-cell transcriptomes of various human cortical brain organoids together with fetal brain to define the identity of specific cell types and differentiation routes in each method. Importantly, we identify unique developmental programs in each protocol compared to fetal brain, which will be a critical benchmark for the utility of human brain organoids in the future.

引用

页码：1682 / +

页数：11

共 41 条

[1] Dissection of Regulatory Networks that Are Altered in Disease via Differential Co-expression [J].

Amar, David ;

Safer, Hershel ;

Shamir, Ron .

PLOS COMPUTATIONAL BIOLOGY, 2013, 9 (03)

[2] Fused cerebral organoids model interactions between brain regions [J].

Bagley, Joshua A. ;

Reumann, Daniel ;

Bian, Shan ;

Levi-Strauss, Julie ;

Knoblich, Juergen A. .

NATURE METHODS, 2017, 14 (07) :743-+

[3] Assembly of functionally integrated human forebrain spheroids [J].

Birey, Fikri ;

Andersen, Jimena ;

Makinson, Christopher D. ;

Islam, Saiful ;

Wei, Wu ;

Huber, Nina ;

Fan, H. Christina ;

Metzler, Kimberly R. Cordes ;

Panagiotakos, Georgia ;

Thom, Nicholas ;

O'Rourke, Nancy A. ;

Steinmetz, Lars M. ;

Bernstein, Jonathan A. ;

Hallmayer, Joachim ;

Huguenard, John R. ;

Pasca, Sergiu P. .

NATURE, 2017, 545 (7652) :54-+

[4] The temporal and spatial origins of cortical interneurons predict their physiological subtype [J].

Butt, SJB ;

Fuccillo, M ;

Nery, S ;

Noctor, S ;

Kriegstein, A ;

Corbin, JG ;

Fishell, G .

NEURON, 2005, 48 (04) :591-604

[5] Engineering of human brain organoids with a functional vascular-like system [J].

Cakir, Bilal ;

Xiang, Yangfei ;

Tanaka, Yoshiaki ;

Kural, Mehmet H. ;

Parent, Maxime ;

Kang, Young-Jin ;

Chapeton, Kayley ;

Patterson, Benjamin ;

Yuan, Yifan ;

He, Chang-Shun ;

Raredon, Micha Sam B. ;

Dengelegi, Jake ;

Kim, Kun-Yong ;

Sun, Pingnan ;

Zhong, Mei ;

Lee, Sangho ;

Patra, Prabir ;

Hyder, Fahmeed ;

Niklason, Laura E. ;

Lee, Sang-Hun ;

Yoon, Young-Sup ;

Park, In-Hyun .

NATURE METHODS, 2019, 16 (11) :1169-+

[6] The single-cell transcriptional landscape of mammalian organogenesis [J].

Cao, Junyue ;

Spielmann, Malte ;

Qiu, Xiaojie ;

Huang, Xingfan ;

Ibrahim, Daniel M. ;

Hill, Andrew J. ;

Zhang, Fan ;

Mundlos, Stefan ;

Christiansen, Lena ;

Steemers, Frank J. ;

Trapnell, Cole ;

Shendure, Jay .

NATURE, 2019, 566 (7745) :496-+

[7] Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling [J].

Chambers, Stuart M. ;

Fasano, Christopher A. ;

Papapetrou, Eirini P. ;

Tomishima, Mark ;

Sadelain, Michel ;

Studer, Lorenz .

NATURE BIOTECHNOLOGY, 2009, 27 (03) :275-280

[8] A survey of human brain transcriptome diversity at the single cell level [J].

Darmanis, Spyros ;

Sloan, Steven A. ;

Zhang, Ye ;

Enge, Martin ;

Caneda, Christine ;

Shuer, Lawrence M. ;

Gephart, Melanie G. Hayden ;

Barres, Ben A. ;

Quake, Stephen R. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (23) :7285-7290

[9] Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals [J].

Eiraku, Mototsugu ;

Watanabe, Kiichi ;

Matsuo-Takasaki, Marni ;

Kawada, Masako ;

Yonemura, Shigenobu ;

Matsumura, Michiru ;

Wataya, Takafumi ;

Nishiyama, Ayaka ;

Muguruma, Keiko ;

Sasail, Yoshiki .

CELL STEM CELL, 2008, 3 (05) :519-532

[10] Efficient Differentiation of Human Embryonic and Induced Pluripotent Stem Cells into Functional Astrocytes [J].

Emdad, Luni ;

D'Souza, Sunita L. ;

Kothari, Harini P. ;

Qadeer, Zulekha A. ;

Germano, Isabelle M. .

STEM CELLS AND DEVELOPMENT, 2012, 21 (03) :404-410

← 1 2 3 4 5 →