Microsatellite DNA loci from the Diamondback terrapin (Malaclemys terrapin)

被引：110

作者：

Hauswaldt, JS ^{[1
]}

Glenn, TC

机构：

[1] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 USA

[2] Savannah River Ecol Lab, Aiken, SC 29802 USA

来源：

MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 02期

关键词：

dinucleotide repeats; Malaclemys terrapin; microsatellites; primer; SSR; tetranucleotide repeats;

D O I：

10.1046/j.1471-8286.2003.00388.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe polymerase chain recation (PCR) primers and conditions to amplify one dinucleotide and five tetranucleotide microsatellite DNA loci isolated from the Diamondback terrapin (Malaclemys terrapin). The PCR primers were tested on 21 terrapins from Cape Romain, SC, USA. The microsatellite primers developed yielded a high number of alleles (8-14) and high observed heterozygosities (0.57-1.0).

引用

页码：174 / 176

页数：3

共 10 条

[1] M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods [J].

Boutin-Ganache, I ;

Raposo, M ;

Raymond, M ;

Deschepper, CF .

BIOTECHNIQUES, 2001, 31 (01) :24-+

[2]

Davis LM, 2001, CROCODILIAN BIOLOGY AND EVOLUTION, P38

[3] TOUCHDOWN PCR TO CIRCUMVENT SPURIOUS PRIMING DURING GENE AMPLIFICATION [J].

DON, RH ;

COX, PT ;

WAINWRIGHT, BJ ;

BAKER, K ;

MATTICK, JS .

NUCLEIC ACIDS RESEARCH, 1991, 19 (14) :4008-4008

[4]

Gibbons J. Whitfield, 2001, Chelonian Conservation and Biology, V4, P66

[5] Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites [J].

Hamilton, MB ;

Pincus, EL ;

Di Fiore, A ;

Fleischer, RC .

BIOTECHNIQUES, 1999, 27 (03) :500-+

[6] GENEPOP (VERSION-1.2) - POPULATION-GENETICS SOFTWARE FOR EXACT TESTS AND ECUMENICISM [J].

RAYMOND, M ;

ROUSSET, F .

JOURNAL OF HEREDITY, 1995, 86 (03) :248-249

[7]

Roosenburg Willem M., 1999, Chelonian Conservation and Biology, V3, P425

[8]

Roosenburg WM, 1996, AM ZOOL, V36, P157

[9]

Sambrook J., 2002, MOL CLONING LAB MANU

[10] Estimates of adult survival and migration for diamondback terrapins: conservation insight from local extirpation within a metapopulation [J].

Tucker, AD ;

Gibbons, JW ;

Greene, JL .

CANADIAN JOURNAL OF ZOOLOGY-REVUE CANADIENNE DE ZOOLOGIE, 2001, 79 (12) :2199-2209

← 1 →