Ameliorative effect of Homalium zeylanicum against carbon tetrachloride-induced oxidative stress and liver injury in rats

被引:14
作者
Kanhar, Satish [1 ]
Sahoo, Atish Kumar [1 ]
机构
[1] Govt Odisha, Reg Plant Resource Ctr, Med & Aromat Plant Div, Phytotherapy Res Lab,Forest & Environm Dept, Bhubaneswar 751015, Odisha, India
关键词
Homalium zeylanicum; ORAC; CAP-e; Antioxidant; serum marker; Hepatic marker; HEPATOPROTECTIVE ACTIVITIES; INDUCED HEPATOTOXICITY; ANTIOXIDANT PROPERTIES; ETHANOLIC EXTRACTS; FREE-RADICALS; GLUTATHIONE; TOXICITY; CAPACITY; METALS; LEAVES;
D O I
10.1016/j.biopha.2018.12.045
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Objective is to evaluate the ameliorative effects of Homalium zeylanicum in carbon tetrachloride-induced oxidative stress and liver injury in rats. To establish the nature of antioxidant principles in the bioactive ethyl acetate fractions of bark (HZEB) and leaf (HZEL); oxygen radical absorbance capacity (ORAC) and cell-based antioxidant protection in erythrocytes (CAP-e) assays were performed. From acute toxicity study, HZEB and HZEL at 200 and 300 mg/kg b.w., were relatively safe at their effective doses. The degree of protection was measured by using biochemical parameters such as serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), total bilirubin (TB) and total protein (TP) contents. Hepatic markers e.g. thiobarbituric acid reactive species (TBARS), superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH) were evaluated along with histopathological observations of liver tissues. Both fractions showed significant improvement in restoring SGOT, SGPT, ALP, TB and TP level. TBARS, SOD, CAT and GSH levels were significantly altered towards normal values. Both fractions at 300 mg/kg showed remarkable improvement in liver markers as compared to silymarin. Histopathological examinations showed reduction in hepatic necrosis and appeared normal hepatocellular architecture in HZEB and HZEL treated groups. In CAP-e assay, IC50 of HZEB (54.66 mg/mL) was higher than HZEL (60.88 mg/mL) and in ORAC assay, AUC of HZEB and HZEL were 33.46, 21.29 respectively and results were comparable with trolox. GC-MS and LC-MS analysis identified a total no. of 44 compounds. Few compounds were identified as bioactive compounds e.g. catechol (7.23%), tetraacetyl-d-xylonic nitrile (3%), oleic acid (0.49%), 2,6-bis(1,1-dimethylethyl)-phenol (3.71%), 3,4,5-trimethoxy-phenol (0.31%), and conifer alcohol (7.41%). The presence of antioxidant principles in both fractions were responsible for hepatoprotective activities, however, the presence of catechol (7.23%) in the bark part imparted better activities in protecting liver than leaf of H. zeylanicum.
引用
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页码:305 / 314
页数:10
相关论文
共 56 条
[1]  
[Anonymous], [No title captured]
[2]   FREE-RADICALS IN TOXICOLOGY [J].
AUST, SD ;
CHIGNELL, CF ;
BRAY, TM ;
KALYANARAMAN, B ;
MASON, RP .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 1993, 120 (02) :168-178
[3]   Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal [J].
Ayala, Antonio ;
Munoz, Mario F. ;
Argueelles, Sandro .
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2014, 2014
[4]   ALTERED THIOL AND CALCIUM HOMEOSTASIS IN OXIDATIVE HEPATOCELLULAR INJURY [J].
BELLOMO, G ;
ORRENIUS, S .
HEPATOLOGY, 1985, 5 (05) :876-882
[5]  
Bendary E., 2013, Annals of Agricultural Science (Cairo), V58, P173, DOI 10.1016/j.aoas.2013.07.002
[6]   OXYGEN-RADICAL ABSORBENCY CAPACITY ASSAY FOR ANTIOXIDANTS [J].
CAO, GH ;
ALESSIO, HM ;
CUTLER, RG .
FREE RADICAL BIOLOGY AND MEDICINE, 1993, 14 (03) :303-311
[7]   Determination of Phenolic Acids and Flavonoids in Taraxacum formosanum Kitam by Liquid Chromatography-Tandem Mass Spectrometry Coupled with a Post-Column Derivatization Technique [J].
Chen, Hung-Ju ;
Inbaraj, Baskaran Stephen ;
Chen, Bing-Huei .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2012, 13 (01) :260-285
[8]   MECHANISMS OF CARBON-TETRACHLORIDE HEPATOTOXICITY [J].
CLAWSON, GA .
PATHOLOGY AND IMMUNOPATHOLOGY RESEARCH, 1989, 8 (02) :104-112
[9]   RESULTS OF A CONTROLLED CLINICAL TRIAL OF GLUTATHIONE IN CASES OF HEPATIC CIRRHOSIS [J].
COOK, GC ;
SHERLOCK, S .
GUT, 1965, 6 (05) :472-&
[10]  
Cuzzocrea S, 2001, PHARMACOL REV, V53, P135