Inhibition effects of low concentrations of epigallocatechin gallate on the biofilm formation and hemolytic activity of Listeria monocytogenes

被引:65
|
作者
Du, Wenfang [1 ]
Zhou, Min [1 ]
Liu, Zhiguo [1 ]
Chen, Ying [2 ]
Li, Rui [1 ]
机构
[1] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China
[2] Chinese Acad Inspect & Quarantine, Beijing 100176, Peoples R China
关键词
EGCG; L; monocytogenes; Biofilm; Virulence; Inhibition; FOOD; EXPRESSION; ENVIRONMENTS; VIRULENCE; STRAINS; TEMPERATURES; PERSISTENT; RESISTANCE; MOTILITY; CHITOSAN;
D O I
10.1016/j.foodcont.2017.09.011
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Listeria monocytogenes (L. monocytogenes) is a well-known food-borne pathogen that causes systemic listeriosis. Its biofilm-forming ability is known to be important for its antimicrobial resistance and persistence. Epigallocatechin-gallate (EGCG) is the highest component of tea polyphenols in tea extracts and has broad-spectrum antimicrobial activities. In this study, the efficacys of EGCG to inhibit biofilm formation and hemolytic activity of L. monocytogenes were determined. EGCG at 20 mu g/mL, 40 mu g/mL (less than compound's minimum inhibitory concentration, MIC) and 200 mu g/mL (1 MIC) were tested. Crystal violet staining showed that sub-MIC and MIC of EGCG could significantly reduce the biofilm formation by L. monocytogenes on polystyrene microtiter plates at three temperatures (15 degrees C, 30 degrees C and 37 degrees C). EGCG reduced the sessile cell numbers present in biofilm at those temperatures. EGCG also significantly inhibited hemolytic activity of L. monocytogenes as measured by sheep red blood cells. Real time PCR assay was used to investigate the relative gene expression of L monocytogenes grown at 37 degrees C and revealed that EGCG down-regulated virulence genes (inlA and hly), SOS response genes (recA and yneA) and quorum sensing gene (agrA). These results suggested the feasibility of using EGCG in food industry to control L monocytogenes biofilm formation. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码:119 / 126
页数:8
相关论文
共 50 条
  • [41] Exploring inhibition of Listeria monocytogenes biofilm by carvacrol based on action to quorum sensing
    Gao, Xiaona
    Gao, Xinke
    Hua, Zichun
    Alhomrani, Majid
    Shi, Chao
    Lin, Lin
    Zhu, Yulin
    FOOD BIOSCIENCE, 2025, 63
  • [42] Growth, biofilm formation, and motility of Listeria monocytogenes strains isolated from food and clinical samples located in Shanghai (China)
    Gao, Binru
    Cai, Hua
    Xu, Biyao
    Yang, Fan
    Dou, Xin
    Dong, Qingli
    Yan, Hui
    Bu, Xiangfeng
    Li, Zhuosi
    FOOD RESEARCH INTERNATIONAL, 2024, 184
  • [43] Adhesion and kinetics of biofilm formation and related gene expression of Listeria monocytogenes in response to nutritional stress
    Wang, Yuan
    Sun, Linjun
    Hu, Lili
    Wang, Zhen
    Wang, Xiang
    Dong, Qingli
    FOOD RESEARCH INTERNATIONAL, 2022, 156
  • [44] Disruption of lmo1386, a putative DNA translocase gene, affects biofilm formation of Listeria monocytogenes on abiotic surfaces
    Chang, Yuhua
    Gu, Weimin
    Zhang, Fujia
    McLandsborough, Lynne
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2013, 161 (03) : 158 - 163
  • [45] Identification of genes involved in Listeria monocytogenes biofilm formation by mariner-based transposon mutagenesis
    Chang, Yuhua
    Gu, Weimin
    Fischer, Nils
    McLandsborough, Lynne
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 93 (05) : 2051 - 2062
  • [46] A Regulatory SRNA Rli43 Is Involved in the Modulation of Biofilm Formation and Virulence in Listeria monocytogenes
    Wang, Lixia
    Ji, Chunhui
    Xia, Xianzhu
    Cai, Xuepeng
    Meng, Qingling
    Qiao, Jun
    PATHOGENS, 2022, 11 (10):
  • [47] The ribonuclease PNPase is a key regulator of biofilm formation in Listeria monocytogenes and affects invasion of host cells
    Quendera, Ana Patricia
    Pinto, Sandra Nunes
    Pobre, Vania
    Antunes, Wilson
    Bonifacio, Vasco D. B.
    Arraiano, Cecilia Maria
    Andrade, Jose Marques
    NPJ BIOFILMS AND MICROBIOMES, 2023, 9 (01)
  • [48] In Vitro Evaluation of Bacteriocins Activity Against Listeria monocytogenes Biofilm Formation
    Anderson Carlos Camargo
    Otávio Almeida Lino de Paula
    Svetoslav Dimitrov Todorov
    Luís Augusto Nero
    Applied Biochemistry and Biotechnology, 2016, 178 : 1239 - 1251
  • [49] Effects of RNase III rncS Gene Deletion on Stress Response, Biofilm Formation and Virulence of Listeria monocytogenes
    Wang, Lixia
    Qiao, Mengfan
    Meng, Qingling
    Qiao, Jun
    Wu, Yehui
    Guo, Jing
    Wang, Xifeng
    Li, Jie
    Zhang, Xingxing
    Cai, Xuepeng
    KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, 2020, 26 (02) : 269 - 277
  • [50] The synergistic antibacterial effect and inhibition of biofilm formation of nisin in combination with terpenes against Listeria monocytogenes
    Shi, D. L.
    Shi, H.
    LETTERS IN APPLIED MICROBIOLOGY, 2022, 75 (03) : 632 - 642