RP-HPLC-DAD analysis of phenolic compounds in pomace extracts from five grape cultivars: Evaluation of their antioxidant, antiradical and antifungal activities in orange and apple juices

被引：102

作者：

Sagdic, Osman ^{[1
]}

Ozturk, Ismet ^{[1
]}

Ozkan, Gulcan ^{[2
]}

Yetim, Hasan ^{[1
]}

Ekici, Lutfiye ^{[1
]}

Yilmaz, Mustafa Tahsin ^{[3
]}

机构：

[1] Erciyes Univ, Fac Engn, Dept Food Engn, TR-38039 Kayseri, Turkey

[2] Suleyman Demirel Univ, Fac Engn, Dept Food Engn, TR-32260 Isparta, Turkey

[3] Erciyes Univ, Safiye Cikrikcioglu Vocat Coll, Dept Food Technol, TR-38039 Kayseri, Turkey

来源：

FOOD CHEMISTRY | 2011年 / 126卷 / 04期

关键词：

Grape pomace extracts; Phenolic compounds; RP-HPLC; Antioxidant activity; Antifungal activity in vitro and in situ;

D O I：

10.1016/j.foodchem.2010.12.075

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Phenolic compounds, related to antioxidative and antifungal properties of ethanolic extracts from five commercial grape cultivars (three red and two white) grown in Turkey were determined. A reversed-phase high performance liquid chromatography (RP-HPLC) procedure was developed, and a total 18 different phenolic compounds were identified. Total phenolic contents of the extracts were determined using Folin-Ciocalteau method. Antioxidant activities of the extracts were evaluated by using DPPH radical scavenging and phosphomolybdenum methods. All extracts exhibited strong antioxidant and antiradical activity. Phenolic compounds and antioxidant activities of the extracts were variety dependent. Antifungal activities of the pomaces and extracts were screened by both in vitro agar-well diffusion assay and antifungal activity in apple and orange juices in situ using Zygosaccharomyces rouxii and Z bailii. Antifungal activities revealed that the pomaces and extracts of Gamay and Kalecik karasi could be more effective antifungal agents than those of Emir, Narince and Okuzgozu grape cultivars. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1749 / 1758

页数：10

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