Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp

被引：70

作者：

Sullivan, Timothy J. ^{[1
]}

Dhar, Arun K. ^{[2
]}

Cruz-Flores, Roberto ^{[2
]}

Bodnar, Andrea G. ^{[1
]}

机构：

[1] Gloucester Marine Genom Inst, Gloucester, MA 01930 USA

[2] Univ Arizona, Sch Anim & Comparat Biomed Sci, Aquaculture Pathol Lab, Tucson, AZ 85721 USA

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

NUCLEIC-ACID DETECTION; AQUACULTURE; WSSV; PCR;

D O I：

10.1038/s41598-019-56170-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Rapid, sensitive, point-of-care diagnostics are critical for managing infectious diseases. Here we adapt the CRISPR-based SHERLOCK method to develop a rapid, accurate, single copy detection assay for White Spot Syndrome Virus, the most devastating virus impacting global shrimp aquaculture. Further, we combine paper matrix nucleic acid extraction and lateral flow colorimetric reporting to create a fully field-deployable, next-generation diagnostic with potential to transform veterinary pathology, disease ecology, and animal production.

引用

页数：7

共 26 条

[1] Detecting pathogens with Zinc-Finger, TALE and CRISPR- based programmable nucleic acid binding proteins [J].

Batista, Angelo C. ;

Pacheco, Luis G. C. .

JOURNAL OF MICROBIOLOGICAL METHODS, 2018, 152 :98-104

[2] Aquaculture: global status and trends [J].

Bostock, John ;

McAndrew, Brendan ;

Richards, Randolph ;

Jauncey, Kim ;

Telfer, Trevor ;

Lorenzen, Kai ;

Little, David ;

Ross, Lindsay ;

Handisyde, Neil ;

Gatward, Iain ;

Corner, Richard .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2010, 365 (1554) :2897-2912

[3] Potential application of CRISPR-Cas12a fluorescence assay coupled with rapid nucleic acid amplification for detection of white spot syndrome virus in shrimp [J].

Chaijarasphong, Thawatchai ;

Thammachai, Thanyawit ;

Itsathitphaisarn, Ornchuma ;

Sritunyalucksana, Kallaya ;

Suebsing, Rungkarn .

AQUACULTURE, 2019, 512

[4]

Cheng Qing-yu, 2007, Virologica Sinica, V22, P61

[5] Quantitative real time PCR for the measurement of white spot syndrome virus in shrimp [J].

Durand, SV ;

Lightner, DV .

JOURNAL OF FISH DISEASES, 2002, 25 (07) :381-389

[6] A future vision for disease control in shrimp aquaculture [J].

Flegel, Timothy W. .

JOURNAL OF THE WORLD AQUACULTURE SOCIETY, 2019, 50 (02) :249-266

[7] Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6 [J].

Gootenberg, Jonathan S. ;

Abudayyeh, Omar O. ;

Kellner, Max J. ;

Joung, Julia ;

Collins, James J. ;

Zhang, Feng .

SCIENCE, 2018, 360 (6387) :439-+

[8] Dehydrogenation of Formic Acid Catalyzed by a Ruthenium Complex with an N,N′-Diimine Ligand [J].

Guan, Chao ;

Zhang, Dan-Dan ;

Pan, Yupeng ;

Iguchi, Masayuki ;

Ajitha, Manjaly J. ;

Hu, Jinsong ;

Li, Huaifeng ;

Yao, Changguang ;

Huang, Mei-Hui ;

Ming, Shixiong ;

Zheng, Junrong ;

Himeda, Yuichiro ;

Kawanami, Hajime ;

Huang, Kuo-Wei .

INORGANIC CHEMISTRY, 2017, 56 (01) :438-445

[9]

KALAGAYAN H, 1991, Journal of the World Aquaculture Society, V22, P235, DOI 10.1111/j.1749-7345.1991.tb00740.x

[10] MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform [J].

Katoh, K ;

Misawa, K ;

Kuma, K ;

Miyata, T .

NUCLEIC ACIDS RESEARCH, 2002, 30 (14) :3059-3066

← 1 2 3 →