A case of 'blown pack' spoilage of vacuum-packaged pork likely associated with Clostridium estertheticum in Canada

This study investigated the potential causative agents for vacuum-packaged pork that had shown gross package extension during a routine storage life study in a Canadian pork plant using both conventional and culture-independent methods. The spoilage-associated bacteria in purge samples from two packages were enumerated using selective media and profiled using 16S rDNA amplicon analysis. The presence of Clostridium estertheticum was detected using species-specific real-time PCR. An enrichment procedure was used to isolate C. estertheticum from one of the purge samples. The average population density in the two purge samples of total aerobes, lactic acid bacteria (LAB), coliforms and Brochothrix thermosphacta was 9.4, 9.1, 6.0 and 4.6 log CFU per ml respectively, as determined by plating. The estimated numbers of C. estertheticum were > 7 log cells per ml. Clostridium estertheticum was recovered although the enrichment condition used for isolation favoured the growth of LAB more than that of Clostridium spp. Based on 16S rDNA amplicon analysis, the microbiota in the two purge samples had 64.7 and 20.7% of Clostridium spp., and 32.5 and 70.1% of LAB respectively.