Jones oxidation and high performance liquid chromatographic analysis of cholesterol in biological samples

被引：23

作者：

Dong, Jun

Chen, Wenxiang ^{[1
]}

Wang, Shu

Zhang, Jiangtao

Li, Hongxia

Guo, Hanbang

Man, Yong

Chen, Baosheng

机构：

[1] Beijing Hosp, Inst Geriatr, Beijing 100730, Peoples R China

[2] Peking Union Med Coll, Beijing 100710, Peoples R China

[3] Chinese Acad Med Sci, Beijing 100710, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 858卷 / 1-2期

关键词：

cholesterol; cholest-4-en-3,6-dione; stigmasterol; chromic acid; HPLC;

D O I：

10.1016/j.jchromb.2007.08.038

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple pre-column derivatization procedure for HPLC analysis of cholesterol in biological samples was developed. Cholesterol was treated with chromic acid and sulfuric acid in acetone (the Jones oxidation) and cholest-4-en-3,6-dione was formed. The reaction was finished in 5 min at room temperature and the product showed a strong UV absorbance at 250 nm that enabled an HPLC detection limit of 0.2 pmol. With stigmasterol as an internal standard, the reaction was applied to the analysis of total and free cholesterol in serum and high-density lipoproteins and the analysis showed a within-run and total coefficient of variation of about 0.2% and 0.5%, respectively. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：239 / 246

页数：8

共 15 条

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