Infrared Emission Spectroscopy for Investigation of Biological Molecules in Aqueous Solutions

被引：7

作者：

Penkov, N., V ^{[1
]}

Penkova, N. A. ^{[2
]}

机构：

[1] Russian Acad Sci, Inst Cell Biophys, Fed Res Ctr, Pushchino Sci Ctr Biol Res, Pushchino 142290, Moscow Oblast, Russia

[2] Russian Acad Sci, Inst Theoret & Expt Biophys, Pushchino 142290, Moscow Oblast, Russia

来源：

PHYSICS OF WAVE PHENOMENA | 2021年 / 29卷 / 02期

关键词：

IR; infrared spectroscopy; emission spectroscopy; protein; phospholipid; ATP; aqueous solution; emission of solution; biological macromolecules; FTIR SPECTROSCOPY; SPECTRA; BACTERIORHODOPSIN; ATMOSPHERE; BILAYERS; PROTEIN; WATER;

D O I：

10.3103/S1541308X21020102

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

Examples are given of using infrared emission spectroscopy to analyze the structure of biological molecules in aqueous solutions. Instead of sample heating commonly used in emission spectroscopy, it is proposed to use a cold background for screening environmental thermal emission. Emission spectra are measured for three types of molecular biological objects in aqueous solutions: protein (bovine serum albumin 1 mg/mL in H2O), phospholipid (1,2-dimiristoylphosphatidylcholine 1 mg/mL in D2O) in two phase states (gel at 10 degrees C and liquid crystal at 35 degrees C), and nucleotide (adenosine-5'-triphosphate 1 mg/mL in H2O). Characteristic bands are obtained for each of the samples. Higher sensitivity and wider application of infrared emission spectroscopy with a cold background in comparison to that with sample heating are discussed.

引用

页码：164 / 168

页数：5

共 25 条

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