High-speed focal modulation microscopy using acousto-optical modulators

被引：15

作者：

Chong, Shau Poh ^{[1
]}

Wong, Chee Howe ^{[1
]}

Wong, Kit Fei ^{[1
]}

Sheppard, Colin J. R. ^{[1
,2
]}

Chen, Nanguang ^{[1
]}

机构：

[1] Natl Univ Singapore, Div Bioengn, Singapore 117576, Singapore

[2] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

来源：

BIOMEDICAL OPTICS EXPRESS | 2010年 / 1卷 / 03期

关键词：

D O I：

10.1364/BOE.1.001026

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Focal Modulation Microscopy (FMM) is a single-photon excitation fluorescence microscopy technique which effectively rejects the out-of-focus fluorescence background that arises when imaging deep inside biological tissues. Here, we report on the implementation of FMM in which laser intensity modulation at the focal plane is achieved using acousto-optic modulators (AOM). The modulation speed is greatly enhanced to the MHz range and thus enables real-time image acquisition. The capability of FMM is demonstrated by imaging fluorescence labeled vasculatures in mouse brain as well as self-made tissue phantom. (C) 2010 Optical Society of America

引用

页码：1026 / 1033

页数：8

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