Interleukin 4 increases CCR9 expression and homing of lymphocytes to gut-associated lymphoid tissue in chickens

被引：8

作者：

Annamalai, Thavamathi ^{[1
]}

Selvaraj, Ramesh K. ^{[1
]}

机构：

[1] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Anim Sci, Wooster, OH 44691 USA

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2012年 / 145卷 / 1-2期

关键词：

IL-4; CCR9; GALT; CHEMOKINE RECEPTOR 9; T-CELLS; DENDRITIC CELLS; OVO; IMMUNIZATION; VACCINATION; THYMOCYTES; KINETICS; VIRUS; TECK;

D O I：

10.1016/j.vetimm.2011.11.016

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The effects of in vitro and in vivo IL-4 supplementation on thymocyte and splenocyte CCR9 mRNA amount and migration were studied. Thymocytes, splenocytes, splenocytes + thymocytes (2:1), and splenocytes + bursocyte cells (2:1) were supplemented with either 0 or 5 ng/ml IL-4 for 5 d. CCR9 mRNA was undetectable in all experimental groups supplemented with 0 ng/ml IL-4. IL-4 treatment (5 ng/ml) upregulated (P = 0.01) CCR9 mRNA only in the splenocyte + thymocyte cell culture. IL-4-mediated CCR9 mRNA induction in the splenocyte + thymocyte cell culture was dependent on the in vitro dose of IL-4 supplementation. IL-4-treated splenocyte + thymocyte cells when injected in vivo preferentially migrated to cecal tonsils. In vivo supplementation of IL-4 was achieved through in ovo injection of recombinant chicken IL-4 plasmid. Cecal tonsils in chicks hatched from IL-4-plasmid-injected eggs weighed more, had a higher amount of CCR9 mRNA, and had a higher percentage of CD8(+) cells than cecal tonsils from chicks hatched from PBS-injected eggs. It could be concluded that IL-4 induces CCR9 mRNA in thymocytes and splenocytes and directs the migration of cells to gut-associated lymphoid tissue. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：257 / 263

页数：7

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