Fluence Plays a Critical Role on the Subsequent Distribution of Chemotherapy and Tumor Growth Delay in Murine Mesothelioma Xenografts Pre-Treated by Photodynamic Therapy

被引：7

作者：

Wang, Yabo ^{[1
]}

Wang, Xingyu ^{[1
]}

Le Bitoux, Marie-Aude ^{[2
]}

Wagnieres, Georges ^{[3
]}

Vandenbergh, Hubert ^{[3
]}

Gonzalez, Michel ^{[1
]}

Ris, Hans-Beat ^{[1
]}

Perentes, Jean Y. ^{[1
]}

Krueger, Thorsten ^{[1
]}

机构：

[1] CHU Vaudois, Ecole Polytech Fed Lausanne, Dept Thorac & Vasc Surg, CH-1011 Lausanne, Switzerland

[2] CHU Vaudois, Ecole Polytech Fed Lausanne, Dept Pathol, CH-1011 Lausanne, Switzerland

[3] Ecole Polytech Fed Lausanne, Dept Chem, Lausanne, Switzerland

来源：

LASERS IN SURGERY AND MEDICINE | 2015年 / 47卷 / 04期

关键词：

photodynamic therapy; drug/light conditions; tumor response; mesothelioma lipoplatin (R); NEAR-INFRARED LIGHT; LEVEL LASER THERAPY; TRAUMATIC BRAIN-INJURY; TERM NEUROLOGICAL DEFICITS; ADULT HEAD MODEL; TRANSCRANIAL LASER; OPTICAL-PROPERTIES; EMBOLIC STROKES; ISCHEMIC-STROKE; MICE;

D O I：

10.1002/lsm.22329

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Background: The pre-conditioning of tumor vessels by low-dose photodynamic therapy (L-PDT) was shown to enhance the distribution of chemotherapy in different tumor types. However, how light dose affects drug distribution and tumor response is unknown. Here we determined the effect of L-PDT fluence on vascular transport in human mesothelioma xenografts. The best L-PDT conditions regarding drug transport were then combined with Lipoplatin (R) to determine tumor response. Methods: Nude mice bearing dorsal skinfold chambers were implanted with H-Meso1 cells. Tumors were treated by Visudyne (R)-mediated photodynamic therapy with 100 mW/cm(2) fluence rate and a variable fluence (5, 10, 30, and 50 J/cm(2)). FITC-Dextran (FITC-D) distribution was assessed in real time in tumor and normal tissues. Tumor response was then determined with best L-PDT conditions combined to Lipoplatin (R) and compared to controls in luciferase expressing H-Meso1 tumors by size and whole body bioluminescence assessment (n = 7/group). Results: Tumor uptake of FITC-D following L-PDT was significantly enhanced by 10-fold in the 10 J/cm(2) but not in the 5, 30, and 50 J/cm(2) groups compared to controls. Normal surrounding tissue uptake of FITC-D following L-PDT was significantly enhanced in the 30 J/cm(2) and 50 J/cm(2) groups compared to controls. Altogether, the FITC-D tumor to normal tissue ratio was significantly higher in the 10 J/cm(2) group compared others. Tumor growth was significantly delayed in animals treated by 10 J/cm2-L-PDT combined to Lipoplatin (R) compared to controls. Conclusions: Fluence of L-PDT is critical for the optimal distribution and effect of subsequently administered chemotherapy. These findings have an importance for the clinical translation of the vascular L-PDT concept in the clinics. Lasers Surg. Med. 47:323-330, 2015. (C) 2015 Wiley Periodicals, Inc.

引用

页码：323 / 330

页数：8

共 1 条

[1] Light dose plays a critical role for the distribution of chemotherapy and tumor response in mesothelioma xenografts pre-treated by photodynamic therapy
Perentes, J. Y.
Wang, X.
Wang, Y.
Le Bitoux, M. -A.
Wagnieres, G.
Vandenbergh, H.
Gonzalez, M.
Ris, H. -B.
Krueger, T.
BRITISH JOURNAL OF SURGERY, 2014, 101 : 23 - 23

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