Silymarin efficacy against influenza A virus replication

被引：36

作者：

Song, J. H. ^{[2
]}

Choi, H. J. ^{[1
]}

机构：

[1] Daejeon Hlth Sci Coll, Taejon 300711, South Korea

[2] Wonkwang Univ, Profess Grad Sch Oriental Med, Dept Herbal Resources, Iksan 570749, South Korea

来源：

PHYTOMEDICINE | 2011年 / 18卷 / 10期

关键词：

Silymarin; Antiviral; Influenza; RT-PCR; DRUG;

D O I：

10.1016/j.phymed.2011.01.026

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The anti-influenza A/PR/8/34 virus activity of silymarin was evaluated in MDCK cells and investigated the effect of silymarin on synthesis of viral mRNAs. Silymarin was investigated for its antiviral activity against influenza A/PR virus using a cytopathic effect (CPE) reduction method. Silymarin exhibited anti-influenza A/PR/8/34 virus activity of 98% with no cytotoxicity at the concentration of 100 mu g/ml reducing the formation of a visible CPE. Oseltamivir also did show moderate antiviral activity of about 52% against influenza A/PR/8/34 virus at the concentration of 100 mu g/ml. Furthermore, the mechanism of anti-influenza virus action in the inhibition of viral mRNA synthesis was analyzed by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR), and the data indicated an inhibitory effect in late viral RNA synthesis compared with oseltamivir in the presence of 100 mu g/ml of silymarin. Therefore, the potential of silymarin for use in treating influenza virus infection merits greater attention. (C) 2011 Elsevier GmbH. All rights reserved.

引用

页码：832 / 835

页数：4

共 16 条

[1] Current and future antiviral therapy of severe seasonal and avian influenza [J].

Beigel, John ;

Bray, Mike .

ANTIVIRAL RESEARCH, 2008, 78 (01) :91-102

[2] Inhibitory effects of quercetin 3-rhamnoside on influenza A virus replication [J].

Choi, Hwa Jung ;

Song, Jae Hyoung ;

Park, Kwi Sung ;

Kwon, Dur Han .

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2009, 37 (3-4) :329-333

[3] Antiviral activity of quercetin 7-rhamnoside against porcine epidemic diarrhea virus [J].

Choi, Hwa-Jung ;

Kim, Jin-Hee ;

Lee, Choong-Hwan ;

Ahn, Young-Joon ;

Song, Jae-Hyoung ;

Baek, Seung-Hwa ;

Kwon, Dur-Han .

ANTIVIRAL RESEARCH, 2009, 81 (01) :77-81

[4]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[5] Detection of human novel influenza A (H1N1) viruses using multi-fluorescent real-time RT-PCR [J].

Dong, Hui ;

Zhang, Yanjun ;

Xiong, Hui ;

Yan, An ;

Ding, Guohui ;

Chen, Yangyi ;

Xie, Liqun ;

Chen, Jiazheng ;

Zhang, Guoqing ;

Hao, Pei ;

Cong, Liming ;

Lu, Yiyu ;

Che, Xiaoyan ;

Wang, Xiaoning ;

Li, Yixue ;

Yuen, Kwok-Yung ;

Zhao, Guoping ;

Jin, Weirong .

VIRUS RESEARCH, 2010, 147 (01) :85-90

[6] Controlling influenza by inhibiting the virus's neuraminidase [J].

Garman, E ;

Laver, G .

CURRENT DRUG TARGETS, 2004, 5 (02) :119-136

[7] Perspectives on antiviral use during pandemic influenza [J].

Hayden, FG .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2001, 356 (1416) :1877-1884

[8]

Hayden Frederick G, 2004, Pediatr Infect Dis J, V23, pS262, DOI 10.1097/01.inf.0000144680.39895.ce

[9] Recovery of drug-resistant influenza virus from immunocompromised patients: A case series [J].

Ison, MG ;

Gubareva, LV ;

Atmar, RL ;

Treanor, J ;

Hayden, FG .

JOURNAL OF INFECTIOUS DISEASES, 2006, 193 (06) :760-764

[10] Influenza pandemics of the 20th century [J].

Kilbourne, ED .

EMERGING INFECTIOUS DISEASES, 2006, 12 (01) :9-14

← 1 2 →