Identification and characterization of variable-number tandem-repeat markers for the molecular epidemiological analysis of Mycoplasma mycoides subspecies mycoides SC

被引:13
作者
McAuliffe, Laura [1 ]
Ayling, Roger D. [1 ]
Nicholas, Robin A. J. [1 ]
机构
[1] Vet Lab Agency Weybridge, Dept Statutory & Exotic Bacterial Dis, Mycoplasma Grp, Weybridge KT15 3NB, Surrey, England
关键词
mycoplasma; CBPP; molecular typing; VNTR;
D O I
10.1111/j.1574-6968.2007.00936.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Variable-number tandem-repeat (VNTR) analysis has been developed for the causative agent of contagious bovine pleuropneumonia using the genome of Mycoplasma mycoides subspecies mycoides small colony (M.m.m. SC) PG1. Genome analysis identified 60 VNTRs within the M.m.m. SC genome; however, screening of these VNTRs with a panel of strains identified only three VNTRs that gave allelic variation. Testing of three VNTRs against 39 strains of diverse geographical origin gave 12 different VNTR profiles groups. VNTR analysis may represent a new rapid tool for subtyping M.m.m. SC isolates.
引用
收藏
页码:181 / 188
页数:8
相关论文
共 27 条
[1]   Insertion element IS1296 in Mycoplasma mycoides subsp mycoides small colony identifies a European clonal line distinct from African and Australian strains [J].
Cheng, XX ;
Nicolet, J ;
Poumarat, F ;
Regalla, J ;
Thiaucourt, F ;
Frey, J .
MICROBIOLOGY-UK, 1995, 141 :3221-3228
[2]   Strain typing of Borrelia burgdorferi, Borrelia aftelii, and Borrelia garinii by using multiple-locus variable-number tandem repeat analysis [J].
Farlow, J ;
Postic, D ;
Smith, KL ;
Jay, Z ;
Baranton, G ;
Keim, P .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (12) :4612-4618
[3]   Francisella tularensis strain typing using multiple-locus, variable-number tandem repeat analysis [J].
Farlow, J ;
Smith, KL ;
Wong, J ;
Abrams, M ;
Lytle, M ;
Keim, P .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (09) :3186-3192
[4]   IDENTIFICATION AND CHARACTERIZATION OF IS1296 IN MYCOPLASMA-MYCOIDES SUBSP MYCOIDES SC AND PRESENCE IN RELATED MYCOPLASMAS [J].
FREY, J ;
CHENG, XX ;
KUHNERT, P ;
NICOLET, J .
GENE, 1995, 160 (01) :95-100
[5]   Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats [J].
Frothingham, R ;
Meeker-O'Connell, WA .
MICROBIOLOGY-UK, 1998, 144 :1189-1196
[6]   Antigen heterogeneity among Mycoplasma mycoides subsp. mycoides SC isolates:: discrimination of major surface proteins [J].
Gonçalves, R ;
Regalla, J ;
Nicolet, J ;
Frey, J ;
Nicholas, R ;
Bashiruddin, J ;
de Santis, P ;
Gonçalves, AP .
VETERINARY MICROBIOLOGY, 1998, 63 (01) :13-28
[7]  
Keim P, 2002, CURR TOP MICROBIOL, V271, P21
[8]   Two separate quorum-sensing systems upregulate transcription of the same ABC transporter in Streptococcus pneumoniae [J].
Knutsen, E ;
Ween, O ;
Håvarstein, LS .
JOURNAL OF BACTERIOLOGY, 2004, 186 (10) :3078-3085
[9]   Molecular epidemiology of contagious bovine pleuropneumonia in Tanzania based on amplified fragment length polymorphism and pulsed-field gel electrophoresis analysis [J].
Kusiluka, LJM ;
Ojeniyi, B ;
Friis, NF ;
Kokotovic, B ;
Ahrens, P .
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 2001, 48 (04) :303-312
[10]   Molecular epidemiology of contagious bovine pleuropneumonia by multilocus sequence analysis of Mycoplasma mycoides subspecies mycoides biotype SC strains [J].
Lorenzon, S ;
Arzul, I ;
Peyraud, A ;
Hendrikx, P ;
Thiaucourt, F .
VETERINARY MICROBIOLOGY, 2003, 93 (04) :319-333