Vaccination with Peptide 173-193 of Acetylcholine Receptor α7-Subunit Prevents Memory Loss in Olfactory Bulbectomized Mice

被引:20
作者
Kamynina, Anna V. [1 ]
Volpina, Olga M. [1 ]
Medvinskaya, Natalya I. [2 ]
Aleksandrova, Irina Ju. [2 ]
Volkova, Tatyana D. [1 ]
Koroev, Dmitriy O. [1 ]
Samokhin, Aleksandr N. [2 ]
Nesterova, Inna V. [2 ]
Shelukhina, Irina V. [1 ]
Kryukova, Elena V. [1 ]
Tsetlin, Viktor I. [1 ]
Ivanov, Vadim T. [1 ]
Bobkova, Natalya V. [2 ]
机构
[1] Russian Acad Sci, Inst Bioorgan Chem, Moscow 117997, Russia
[2] Russian Acad Sci, Inst Cell Biophys, Pushchino 142292, Russia
关键词
alpha 7-subunit of the nicotinic acetylcholine receptor; Alzheimer's disease; amyloid-beta; immunization; olfactory bulbectomized mice; peptide fragments; spatial memory; ALPHA-7 NICOTINIC ACETYLCHOLINE; PROTEIN-KINASE-C; ALZHEIMERS-DISEASE; TEMPORAL CORTEX; ADULT-RAT; BRAIN; NEURONS; IMPAIRMENT; ANTIBODIES; BINDING;
D O I
10.3233/JAD-2010-091474
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We studied the ability of four non-conjugated alpha 7-subunit fragments of the nicotinic acetylcholine receptor to induce an immune response and to protect memory in olfactory bulbectomized mice which demonstrate abnormalities similar to Alzheimer's disease (AD). Vaccination only with the alpha 7-subunit fragment 173-193 was shown to rescue spatial memory, to restore the level of alpha 7 acetylcholine receptors in the cortex, and to prevent an increase in the amyloid-beta (A beta) level in brain tissue in these animals. Antibodies against the peptide 173-193 were revealed in blood serum and cerebrospinal liquid in the bulbectomized mice. Passive immunization with mouse blood sera containing antibodies to the peptide 173-193 also restored memory in bulbectomized animals. The observed positive effect of both active and passive immunization with the fragment of alpha 7-subunit on memory of bulbectomized mice provides a new insight into an anti-AD drug design.
引用
收藏
页码:249 / 261
页数:13
相关论文
共 60 条
[1]   Increased level of β-amyloid in the brain of bulbectomized mice [J].
Aleksandrova, IY ;
Kuvichkin, VV ;
Kashparov, IA ;
Medvinskaya, NI ;
Nesterova, IV ;
Lunin, SM ;
Samokhin, AN ;
Bobkova, NV .
BIOCHEMISTRY-MOSCOW, 2004, 69 (02) :176-180
[2]   Indirect modulation by α7 nicotinic acetylcholine receptors of noradrenaline release in rat hippocampal slices:: Interaction with glutamate and GABA systems and effect of nicotine withdrawal [J].
Barik, J ;
Wonnacott, S .
MOLECULAR PHARMACOLOGY, 2006, 69 (02) :618-628
[3]   Alzheimer's disease [J].
Scheltens, Philip ;
De Strooper, Bart ;
Kivipelto, Miia ;
Holstege, Henne ;
Chetelat, Gael ;
Teunissen, Charlotte E. ;
Cummings, Jeffrey ;
van der Flier, Wiesje M. .
LANCET, 2021, 397 (10284) :1577-1590
[4]   Morphofunctional changes in neurons in the temporal cortex of the brain in relation to spatial memory in bulbectomized mice after treatment with mineral ascorbates [J].
Bobkova N.V. ;
Nesterova I.V. ;
Dana R. ;
Dana E. ;
Nesterov V.I. ;
Aleksandrova I.Yu. ;
Medvinskaya N.I. ;
Samokhin A.N. .
Neuroscience and Behavioral Physiology, 2004, 34 (7) :671-676
[5]   Interhemispheric EEG differences in olfactory bulbectomized rats with different cognitive abilities and brain beta-amyloid levels [J].
Bobkova, Natalia ;
Vorobyov, Vasily ;
Medvinskaya, Natalia ;
Aleksandrova, Irina ;
Nesterovaa, Inna .
BRAIN RESEARCH, 2008, 1232 :185-194
[6]   The state of cholinergic structures in forebrain of bulbectomized mice [J].
Bobkova, NV ;
Nesterova, IV ;
Nesterov, VV .
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 2001, 131 (05) :427-431
[7]  
Capurso SA, 1997, J NEUROSCI, V17, P7372
[8]   The alpha-bungarotoxin-binding nicotinic acetylcholine receptor from rat brain contains only the alpha 7 subunit [J].
Chen, DN ;
Patrick, JW .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (38) :24024-24029
[9]  
Chui DH, 2001, J ALZHEIMERS DIS, V3, P231
[10]   Nicotinic receptor abnormalities in Alzheimer's disease [J].
Court, J ;
Martin-Ruiz, C ;
Piggott, M ;
Spurden, D ;
Griffiths, M ;
Perry, E .
BIOLOGICAL PSYCHIATRY, 2001, 49 (03) :175-184