Collection and Extraction of Saliva DNA for Next Generation Sequencing

被引：32

作者：

Goode, Michael R. ^{[1
]}

Cheong, Soo Yeon ^{[1
]}

Li, Ning ^{[1
]}

Ray, William C. ^{[1
,2
]}

Bartlett, Christopher W. ^{[1
,3
]}

机构：

[1] Nationwide Childrens Hosp, Res Inst, Battelle Ctr Math Med, Columbus, OH 43205 USA

[2] Ohio State Univ, Interdisciplinary Grad Program Biophys, Columbus, OH 43210 USA

[3] Ohio State Univ, Dept Pediat, Columbus, OH 43210 USA

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2014年 / 90期

基金：

美国国家卫生研究院;

关键词：

Medicine; Issue; 90; DNA collection; saliva; DNA extraction; Next generation sequencing; DNA purification; DNA; GENOMIC DNA; FRAMEWORK; SAMPLES; NUMBER; RATES; MOUTH;

D O I：

10.3791/51697

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The preferred source of DNA in human genetics research is blood, or cell lines derived from blood, as these sources yield large quantities of high quality DNA. However, DNA extraction from saliva can yield high quality DNA with little to no degradation/fragmentation that is suitable for a variety of DNA assays without the expense of a phlebotomist and can even be acquired through the mail. However, at present, no saliva DNA collection/extraction protocols for next generation sequencing have been presented in the literature. This protocol optimizes parameters of saliva collection/storage and DNA extraction to be of sufficient quality and quantity for DNA assays with the highest standards, including microarray genotyping and next generation sequencing.

引用

页数：7

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