Reverse elution order of β-blockers in chiral separation

In the field of chiral separation, the elution order of the enantiomers is one of the most important phenomena. Although the reversal of enantiomeric elution order is rare, it has certain significance. In the determination of the optical purities, the study of this phenomenon is helpful because it is favorable to elute the minor enantiomeric impurity before the major component. This mini review reports the reversal order of elution of the most important class of the drugs of beta-blockers. This mini review describes the effects of the composition of the mobile phase, pH, and temperature on the reversal order of elution of beta-blockers. The efforts are also made to discuss the possible future perspectives of reversal order of elution.