Monitoring the antiviral effect of alpha interferon on individual cells

被引：31
作者：
Kim, Chon Saeng
Jung, Jong Ha
Wakita, Takaji
Yoon, Seung Kew
Jang, Sung Key
机构：
[1] Pohang Univ Sci & Technol, Dept Life Sci, PBC, Div Mol & Life Sci, Pohang 790784, South Korea
[2] Natl Inst Infect Dis, Dept Virol 2, Shinjuku Ku, Tokyo 1628640, Japan
[3] Catholic Univ Korea, Coll Med, Dept Internal Med, Seoul, South Korea
来源：
JOURNAL OF VIROLOGY | 2007年 / 81卷 / 16期
关键词：
D O I：
10.1128/JVI.02824-06
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
An infectious hepatitis C virus (HCV) cDNA clone (JFH1) was generated recently. However, quantitative analysis of HCV infection and observation of infected cells have proved to be difficult because the yield of HCV in cell cultures is fairly low. We generated infectious HCV clones containing the convenient reporters green fluorescent protein (GFP) and Renilla luciferase in the NS5a-coding sequence. The new viruses responded to antiviral agents in a dose-dependent manner. Responses of individual cells containing HCV to alpha interferon (IFN-alpha) were monitored using GFP-tagged HCV and time-lapse confocal microscopy. Marked variations in the response to IFN-alpha were observed among HCV-containing cells.
引用
收藏
页码：8814 / 8820
页数：7
相关论文
共 18 条
[1]   Recovery, persistence, and sequelae in hepatitis C virus infection: A perspective on long-term outcome [J].
Alter, HJ ;
Seeff, LB .
SEMINARS IN LIVER DISEASE, 2000, 20 (01) :17-35
[2]   Novel cell culture systems for the hepatitis C virus [J].
Bartenschlager, R ;
Lohmann, V .
ANTIVIRAL RESEARCH, 2001, 52 (01) :1-17
[3]   Efficient hepatitis C virus cell culture system: What a difference the host cell makes [J].
Bartenschlager, R ;
Pietschmann, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (28) :9739-9740
[4]   Mutations in the 5′ nontranslated region of bovine viral diarrhea virus result in altered growth characteristics [J].
Becher, P ;
Orlich, M ;
Thiel, HJ .
JOURNAL OF VIROLOGY, 2000, 74 (17) :7884-7894
[5]   Peginterferon alfa-2a plus ribavirin for chronic hepatitis C virus infection. [J].
Fried, MW ;
Shiffman, ML ;
Reddy, KR ;
Smith, C ;
Marinos, G ;
Goncales, FL ;
Haussinger, D ;
Diago, M ;
Carosi, G ;
Dhumeaux, D ;
Craxi, A ;
Lin, A ;
Hoffman, J ;
Yu, J .
NEW ENGLAND JOURNAL OF MEDICINE, 2002, 347 (13) :975-982
[6]   Treatment of acute hepatitis C with interferon alfa-2b [J].
Jaeckel, E ;
Cornberg, M ;
Wedemeyer, H ;
Santantonio, T ;
Mayer, J ;
Zankel, M ;
Pastore, G ;
Dietrich, M ;
Trautwein, C ;
Manns, MP .
NEW ENGLAND JOURNAL OF MEDICINE, 2001, 345 (20) :1452-1457
[7]  
Kenny-Walsh E, 2001, Clin Liver Dis, V5, P969, DOI 10.1016/S1089-3261(05)70204-X
[8]   Sequestration of TRAF2 into stress granules interrupts tumor necrosis factor signaling under stress conditions [J].
Kim, WJ ;
Back, SH ;
Kim, V ;
Ryu, I ;
Jang, SK .
MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (06) :2450-2462
[9]   Characterization of the early steps of hepatitis C virus infection by using luciferase reporter viruses [J].
Koutsoudakis, George ;
Kaul, Artur ;
Steinmann, Eike ;
Kallis, Stephanie ;
Lohmann, Volker ;
Pietschmann, Thomas ;
Bartenschlager, Ralf .
JOURNAL OF VIROLOGY, 2006, 80 (11) :5308-5320
[10]   An NS3 protease inhibitor with antiviral effects in humans infected with hepatitis C virus [J].
Lamarre, D ;
Anderson, PC ;
Bailey, M ;
Beaulieu, P ;
Bolger, G ;
Bonneau, P ;
Bös, M ;
Cameron, DR ;
Cartier, M ;
Cordingley, MG ;
Faucher, AM ;
Goudreau, N ;
Kawai, SH ;
Kukolj, G ;
Lagacé, L ;
LaPlante, SR ;
Narjes, H ;
Poupart, MA ;
Rancourt, J ;
Sentjens, RE ;
St George, R ;
Simoneau, B ;
Steinmann, G ;
Thibeault, D ;
Tsantrizos, YS ;
Weldon, SM ;
Yong, CL ;
Llinàs-Brunet, M .
NATURE, 2003, 426 (6963) :186-189
12