Amino Acids Supplied through the Autophagy/Endocytosis Pathway Promote Starch Synthesis in Physcomitrella Protonemal Cells

被引:4
作者
Sakil, Md. Arif [1 ,2 ]
Mukae, Kyosuke [1 ,3 ]
Funada, Ryo [1 ]
Kotake, Toshihisa [1 ]
Ueno, Shigeaki [4 ]
Aktar, Most Mohoshena [1 ,5 ]
Roni, Md. Shyduzzaman [1 ,6 ]
Inoue-Aono, Yuko [1 ]
Moriyasu, Yuji [1 ]
机构
[1] Saitama Univ, Grad Sch Sci & Engn, Saitama 3388570, Japan
[2] Bangladesh Agr Univ, Dept Biochem & Mol Biol, Mymensingh 2202, Bangladesh
[3] Saitama Canc Ctr, Res Inst Clin Oncol, Saitama 3620806, Japan
[4] Saitama Univ, Fac Educ, Saitama 3388570, Japan
[5] Hajee Mohammad Danesh Sci & Technol Univ, Dept Agron, Dinajpur 5200, Bangladesh
[6] Bangabandhu Sheikh Mujibur Rahman Agr Univ, Dept Hort, Gazipur 1706, Bangladesh
来源
PLANTS-BASEL | 2022年 / 11卷 / 16期
基金
日本学术振兴会;
关键词
starch synthesis; autophagy; endocytosis; amino acid; Physcomitrella (Physcomitrium); AUTOPHAGY; SENESCENCE; CHLOROPLASTS; PEROXISOMES; VACUOLE; PROTEIN;
D O I
10.3390/plants11162157
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The physiological implications of autophagy in plant cells have not been fully elucidated. Therefore, we investigated the consequences of autophagy in the moss Physcomitrella by measuring biochemical parameters (fresh and dry weights; starch, amino acid, carbohydrate, and NH3 content) in wild-type (WT) and autophagy-deficient atg5 Physcomitrella cells. We found higher starch levels and a higher net starch synthesis rate in WT cells than in atg5 cells cultured in a glucose-containing culture medium, whereas net starch degradation was similar in the two strains cultured in a glucose-deficient culture medium. Additionally, the treatment of cells with the autophagy inhibitor 3-methyladenine suppressed starch synthesis. Loading bovine serum albumin into atg5 cells through endocytosis, i.e., supplying proteins to vacuoles in the same way as through autophagy, accelerated starch synthesis, whereas loading glutamine through the plasma membrane had no such effect, suggesting that Physcomitrella cells distinguish between different amino acid supply pathways. After net starch synthesis, NH3 levels increased in WT cells, although the change in total amino acid content did not differ between WT and atg5 cells, indicating that autophagy-produced amino acids are oxidized rapidly. We conclude that autophagy promotes starch synthesis in Physcomitrella by supplying the energy obtained by oxidizing autophagy-produced amino acids.
引用
收藏
页数:14
相关论文
共 24 条
[1]   Rice BRITTLE CULM 5 (BRITTLE NODE) is Involved in Secondary Cell Wall Formation in the Sclerenchyma Tissue of Nodes [J].
Aohara, Tsutomu ;
Kotake, Toshihisa ;
Kaneko, Yasuko ;
Takatsuji, Hiroshi ;
Tsumuraya, Yoichi ;
Kawasaki, Shinji .
PLANT AND CELL PHYSIOLOGY, 2009, 50 (11) :1886-1897
[2]   Autophagy Deficiency Compromises Alternative Pathways of Respiration following Energy Deprivation in Arabidopsis thaliana [J].
Barros, Jessica A. S. ;
Cavalcanti, Joao Henrique F. ;
Medeiros, David B. ;
Nunes-Nesi, Adriano ;
Avin-Wittenberg, Tamar ;
Fernie, Alisdair R. ;
Araujo, Wagner L. .
PLANT PHYSIOLOGY, 2017, 175 (01) :62-76
[3]  
DE DUVE CHRISTIAN, 1963, SCI AMER, V208, P64
[4]   The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana [J].
Doelling, JH ;
Walker, JM ;
Friedman, EM ;
Thompson, AR ;
Vierstra, RD .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (36) :33105-33114
[5]   A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].
DUBOIS, M ;
GILLES, K ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
NATURE, 1951, 168 (4265) :167-167
[6]   Leaf senescence and starvation-induced chlorosis are accelerated by the disruption of an Arabidopsis autophagy gene [J].
Hanaoka, H ;
Noda, T ;
Shirano, Y ;
Kato, T ;
Hayashi, H ;
Shibata, D ;
Tabata, S ;
Ohsumi, Y .
PLANT PHYSIOLOGY, 2002, 129 (03) :1181-1193
[7]   Vacuolar Protein Degradation via Autophagy Provides Substrates to Amino Acid Catabolic Pathways as an Adaptive Response to Sugar Starvation in Arabidopsis thaliana [J].
Hirota, Takaaki ;
Izumi, Masanori ;
Wada, Shinya ;
Makino, Amane ;
Ishida, Hiroyuki .
PLANT AND CELL PHYSIOLOGY, 2018, 59 (07) :1363-1376
[8]   Mobilization of rubisco and stroma-localized fluorescent proteins of chloroplasts to the vacuole by an ATG gene-dependent autophagic process [J].
Ishida, Hiroyuki ;
Yoshimoto, Kohki ;
Izumi, Masanori ;
Reisen, Daniel ;
Yano, Yuichi ;
Makino, Amane ;
Ohsumi, Yoshinori ;
Hanson, Maureen R. ;
Mae, Tadahiko .
PLANT PHYSIOLOGY, 2008, 148 (01) :142-155
[9]   Establishment of Monitoring Methods for Autophagy in Rice Reveals Autophagic Recycling of Chloroplasts and Root Plastids during Energy Limitation [J].
Izumi, Masanori ;
Hidema, Jun ;
Wada, Shinya ;
Kondo, Eri ;
Kurusu, Takamitsu ;
Kuchitsu, Kazuyuki ;
Makino, Amane ;
Ishida, Hiroyuki .
PLANT PHYSIOLOGY, 2015, 167 (04) :1307-U316
[10]   Cell biology - Autophagy as a regulated pathway of cellular degradation [J].
Klionsky, DJ ;
Emr, SD .
SCIENCE, 2000, 290 (5497) :1717-1721