Antibacterial mechanism of chitosan microspheres in a solid dispersing system against E-coli

被引：258

作者：

Kong, Ming ^{[1
]}

Chen, Xi Guang ^{[1
]}

Liu, Cheng Sheng ^{[1
]}

Liu, Chen Guang ^{[1
]}

Meng, Xiang Hong ^{[1
]}

Yu, Le Jun ^{[1
]}

机构：

[1] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2008年 / 65卷 / 02期

基金：

中国国家自然科学基金;

关键词：

antibacterial; interface; chitosan; microsphere; fluorescence; SEM;

D O I：

10.1016/j.colsurfb.2008.04.003

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this study, we investigated the antibacterial mechanism through the interfacial contacting inhibition behaviors of chitosan antimicrobials against Escherichia coli in solid dispersing state. Chitosan microspheres (CMs) were prepared by emulsification cross-linking reaction, and oleoyl-CMs (OCMs) were obtained by introduction of oleoyl groups to the chitosan. The CMs were with smooth surface and spherical shape of diameter of about 124 mu m. The antibacterial activity was directly proportional to the concentration and the hydrophobic property of CMs. The fluorescence experiments indicated CMs had influenced the structure of membrane, especially the OCMs were speculated to interact with proteins on the cell membrane. SEM photographs showed E. coli adhered to the surface of the CMs and provided evidences for the disruption of the cells, while the bacterium conglomerated on the surface of the OCMs. The CMs changed the permeability of membrane and caused cellular leakage that correlated with the hydrophobic interaction between CMs and cytoplasmic membrane phospholipids of Gram-negative bacteria. Solid dispersing system makes the antibacterial activities of CMs counted as a sequent event-driven to study the antibacterial mechanism of chitosan originally. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：197 / 202

页数：6

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