Molecular cloning and identification of tissue-specific expression of ent-kaurene oxidase gene in Momordica charantia

被引：3

作者：

Lei, Lei ^{[1
]}

Fan, Zheren ^{[1
]}

Tang, Lin ^{[1
]}

Chen, Fang ^{[1
]}

机构：

[1] Sichuan Univ, Minist Educ, Coll Life Sci, Key Lab Bioresources & Ecoenvironm, Chengdu 610064, Peoples R China

来源：

AFRICAN JOURNAL OF BIOTECHNOLOGY | 2011年 / 10卷 / 70期

关键词：

Gibberellin; bioinformatics analysis; procaryotic expression; quantitative real-time polymerase chain reaction qRT-(PCR); GIBBERELLIN BIOSYNTHESIS; IN-VITRO; FRUIT EXTRACT; ANTIOXIDANT; VIVO; L;

D O I：

10.5897/AJB11.1471

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Momordica charantia (bitter melon) is widely consumed as a vegetable and as a folk medicine. Ent-kaurene oxidase is a key enzyme of gibberellin (GA) synthesis by controlling the early GA biosynthesis. In this study, ent-kaurene oxidase cDNA sequence was successfully amplified from the total cDNA of pistillate flower buds, and a complete open reading frame (ORF) with 1563 bp, encoding 520 amino acids, containing 151 bp of 5'-untranslated region sequences and 217 bp of the 3'-untranslated region sequences was found. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed that the expression level of M. charantia ent-kaurene oxidase (McEKO) gene in pistillate flowers was significantly higher than in staminate flowers and neutral flowers, and the expression level was especially abundant in root and stem apexes. The gene was express in Escherichia coli cells with a molecular weight of 58.8 kD observed.

引用

页码：15724 / 15730

页数：7

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