Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1

被引：317
作者：
Lykke-Andersen, J [1 ]
Shu, MD [1 ]
Steitz, JA [1 ]
机构：
[1] Yale Univ, Sch Med, Howard Hughes Med Inst, New Haven, CT 06536 USA
来源：
SCIENCE | 2001年 / 293卷 / 5536期
关键词：
D O I：
10.1126/science.1062786
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In mammalian cells, splice junctions play a dual rote in mRNA quality control: They mediate selective nuclear export of mature mRNA and they serve as a mark for mRNA surveillance, which subjects aberrant mRNAs with premature termination codons to nonsense-mediated decay (NMD). Here, we demonstrate that the protein RNPS1, a component of the postsplicing complex that is deposited 5' to exon-exon junctions, interacts with the evolutionarily conserved human Upf complex, a central component of NMD. Significantly, RNPS1 triggers NMD when tethered to the 3' untranslated region of beta -globin mRNA, demonstrating its role as a subunit of the postsplicing complex directly involved in mRNA surveillance.
引用
收藏
页码：1836 / 1839
页数：4
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