Genomic characterization of a turkey reovirus field strain by Next-Generation Sequencing

被引：22
作者：
Tang, Yi [1 ]
Lu, Huaguang [1 ]
Sebastian, Aswathy [2 ]
Yeh, Yin-Ting [3 ]
Praul, Craig A. [4 ]
Albert, Istvan U. [2 ]
Zheng, Si-Yang [3 ]
机构：
[1] Penn State Univ, Dept Vet & Biomed Sci, Wiley Lab Avian Virol, University Pk, PA 16802 USA
[2] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA
[3] Penn State Univ, Dept Biomed Engn, University Pk, PA 16802 USA
[4] Penn State Univ, Huck Inst Life Sci, Genom Core Facil, University Pk, PA 16802 USA
来源：
INFECTION GENETICS AND EVOLUTION | 2015年 / 32卷
基金：
美国国家卫生研究院; 美国国家科学基金会;
关键词：
Genome segments; Genotyping; Next-Generation Sequencing; Turkey arthritis reovirus; QUAIL COLINUS-VIRGINIANUS; POLYMERASE-CHAIN-REACTION; AVIAN REOVIRUS; PHYLOGENETIC ANALYSIS; INFECTIOUS SYNOVITIS; TENOSYNOVITIS; VIRUS; MORTALITY; ENTERITIS; SEGMENTS;
D O I：
10.1016/j.meegid.2015.03.029
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The genome of a turkey arthritis reovirus (TARV) field strain (Reo/PA/Turkey/22342/13), isolated from a turkey flock in Pennsylvania (PA) in 2013, has been sequenced using Next-Generation Sequencing (NGS) on the Illumina MiSeq platform. The genome of the PA TARV field strain was 23,496 bp in length with 10 dsRNA segments encoding 12 viral proteins. The lengths of the genomic segments ranged from 1192 bp (S4) to 3959 bp (L1). The 5' and 3' conserved terminal sequences of the PA TARV field strain were similar to the two Minnesota (MN) TARVs (MN9 and MN10) published recently and avian orthoreovirus (ARV) reference strains. Phylogenetic analysis of the nucleotide sequences of all 10 genome segments revealed that there was a low to significant nucleotide sequence divergence between the PA TARV field strain and reference TARV and ARV strains. Analysis of the PA TARV sequence indicates that this PA TARV field strain is a unique strain and is different from the TARV MN9 or MN10 in M2 segment genes and ARV S1133 vaccine strain. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：313 / 321
页数：9
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