Glycohydrolases β-hexosaminidase and β-galactosidase are associated with lipid microdomains of Jurkat T-lymphocytes

被引:13
作者
Magini, Alessandro [1 ]
Polchi, Alice [1 ]
Tancini, Brunella [1 ]
Urbanelli, Lorena [1 ]
Hasilik, Andrej [2 ]
Emiliani, Carla [1 ]
机构
[1] Univ Perugia, Dept Expt Med & Biochem Sci, I-06123 Perugia, Italy
[2] Univ Marburg, Inst Physiol Chem, D-35033 Marburg, Germany
关键词
Acidic glycohydrolases; Membrane microdomains; T-lymphocytes; PLASMA-MEMBRANE; IMMUNOLOGICAL SYNAPSE; HUMAN FIBROBLASTS; GANGLIOSIDE GM3; CELL ACTIVATION; FUNCTIONAL-ROLE; RAFTS; GLYCOSPHINGOLIPIDS; IDENTIFICATION; EXPRESSION;
D O I
10.1016/j.biochi.2011.09.021
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Growing evidence suggests the presence of active lysosomal enzymes in extra-lysosomal compartments, such as the plasma membrane. Although in the past little attention was paid to glycohydrolases acting on cellular compartments different from lysosomes, there is now increasing interest on plasma membrane-associated glycohydrolases because they should be involved, together with glycosyltransferases, in glycosphingolipids oligosaccharide modification processes regulating cell-to-cell and/or cell environment interactions in both physiological and pathological conditions. Starting from the previous evidence of the presence of beta-hexosaminidase and beta-galactosidase at the plasma membrane of cultured fibroblasts, we here investigated the association of these glycohydrolases with lipid microdomains of Jurkat T-lymphocytes. Monosialoganglioside GM3 represents the major glycosphingolipid constituent of T-cell plasma membrane and its amount largely increases after T-cell stimulation. beta-hexosaminidase and beta-galactosidase cleave specific beta-linked terminal residues from a wide range of glycoconjugates and in particular are involved in the stepwise degradation of GM1 to GM3 ganglioside. Here we demonstrated that fully processed plasma membrane-associated beta-hexosaminidase and beta-galactosidase co-distribute with the lipid microdomain markers and co-immunoprecipitate with the signalling protein Ick in Jurkat T-cell. Furthermore, Jurkat cell stimulation up-regulates the expression and activity of lysosomal beta-hexosaminidase and beta-galactosidase and increases their targeting to lipid microdomains. The non-random distribution of plasma membrane-associated beta-hexosaminidase and beta-galactosidase and their localization within lipid microdomains, suggest a role of these enzymes in the local reorganization of glycosphingolipid-based signalling units. (C) 2011 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:684 / 694
页数:11
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