Natural abundance 17O NMR spectroscopy of rat brain in vivo

Oxygen is an abundant element that is present in almost all biologically relevant molecules. NMR observation of oxygen has been relatively limited since the NMR-active isotope, oxygen-17, is only present at a 0.037% natural abundance. Furthermore, as a spin 5/2 nucleus oxygen-17 has a moderately strong quadrupole moment which leads to fairly broad resonances (T-2(*) = 1-4 ms). However, the similarly short T-1 relaxation constants allow substantial signal averaging, whereas the large chemical shift range (> 300 ppm) improves the spectral resolution of O-17 NMR. Here it is shown that high-quality, natural abundance O-17 NMR spectra can be obtained from rat brain in vivo at 11.74 T. The chemical shifts and line widths of more than 20 oxygen-containing metabolites are established and the sensitivity and potential for O-17-enriched NMR studies are estimated. (c) 2008 Elsevier Inc. All rights reserved.