Plant regeneration in wheat mature embryo culture

被引：18
作者：
Aydin, Murat [1 ]
Tosun, Metin [1 ]
Haliloglu, Kamil [1 ]
机构：
[1] Ataturk Univ, Dept Field Crops, Fac Agr, Erzurum, Turkey
来源：
AFRICAN JOURNAL OF BIOTECHNOLOGY | 2011年 / 10卷 / 70期
关键词：
Callus formation; explant; genotype; plant growth regulators; TRITICUM-AESTIVUM; CALLUS INDUCTION; TISSUE-CULTURE; IMMATURE EMBRYOS; GENOTYPE; INITIATION; CULTIVARS; EXPLANTS; GROWTH; BARLEY;
D O I：
10.5897/AJB11.1495
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Success in genetic engineering of cereals depends on the callus formation and efficient plant regeneration system. Callus formation and plant regeneration of wheat mature embryos were assessed by using 12 different methods with 4 genotypes. Genotype significantly affected the formation of callus, embryogenic callus and plant regeneration. Only two methods (methods # 2 and 9) produced plant regeneration. The highest plant regeneration for all genotypes with endosperm that supported mature embryos was observed in method # 9 which contain MS medium supplemented with 12 mg/l dicamba + 0.5 mg/l IAA.
引用
收藏
页码：15749 / 15755
页数：7
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