Visualization of protein-RNA interactions in cytoplasmic polyhedrosis virus

被引：67

作者：

Zhang, H

Zhang, J

Yu, X

Lu, X

Zhang, Q

Jakana, J

Chen, DH

Zhang, X

Zhou, ZH ^{[1
]}

机构：

[1] Univ Texas, Sch Med, Dept Pathol & Lab Med, Houston, TX 77030 USA

[2] Baylor Coll Med, Dept Biochem, Houston, TX 77030 USA

[3] Zhongshan Univ, Inst Entomol, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China

[4] Chinese Acad Sci, Inst Phys, Beijing Lab Electron Microscopy, Beijing 100080, Peoples R China

来源：

JOURNAL OF VIROLOGY | 1999年 / 73卷 / 02期

关键词：

D O I：

10.1128/JVI.73.2.1624-1629.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Unlike the multiple-shelled organization of other Reoviridae members, cytoplasmic polyhedrosis virus (CPV) has a single-shelled capsid. The three-dimensional structures of full and empty CPV by electron cryomicroscopy show identical outer shells but differ inside. The outer surface reveals a T=1 icosahedral shell decorated with spikes at its icosahedral vertices. The internal space of the empty CPV is unoccupied except for 12 mushroom-shaped densities attributed to the transcriptional enzyme complexes. The ordered double-stranded RNA inside the full capsid forms spherical shells spaced 25 Angstrom apart. The RNA-protein interactions suggest a mechanism for RNA transcription and release.

引用

页码：1624 / 1629

页数：6

共 33 条

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