Targeting Matrix Metalloproteinases: A Potential Strategy for Improving Cell Transplantation for Nervous System Repair

被引:4
作者
Tseng, Yu-Ting [1 ,2 ,3 ]
Chen, Mo [1 ,2 ,3 ]
John, James St [1 ,2 ,3 ]
Ekberg, Jenny [1 ,2 ,3 ]
机构
[1] Griffith Univ, Menzies Hlth Inst Queensland, Southport, Qld, Australia
[2] Griffith Univ, Clem Jones Ctr Neurobiol & Stem Cell Res, Brisbane, Qld, Australia
[3] Griffith Univ, Griffith Inst Drug Discovery, Brisbane, Qld, Australia
基金
英国医学研究理事会;
关键词
Olfactory ensheathing cells; 3D culture; migration; MMPs; extracellular matrix; spinal cord injury; peripheral nerve; OLFACTORY ENSHEATHING CELLS; SPINAL-CORD-INJURY; CHONDROITIN SULFATE PROTEOGLYCANS; MESENCHYMAL STROMAL CELLS; SCHWANN-CELLS; EXTRACELLULAR-MATRIX; NEURITE EXTENSION; GINSENOSIDE RG1; STEM-CELLS; IGF-I;
D O I
10.1177/09636897211012909
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Cell transplantation shows promise for repair of the injured nervous system, including spinal cord injury (SCI) and peripheral nerve injury (PNI). There are, however, still problems hampering these therapies moving from bench to bedside, and the methods need optimization. Three-dimensional (3D) cell culture systems are suggested to improve outcomes, bridging the gap between the in vitro and in vivo environments. In such constructs, cells are allowed to interact with each other and with the extracellular matrix (ECM) in 3D as they do in vivo. Transplanting cells in 3D constructs, rather than in suspension, is thought to promote cell survival and maintain important cellular behaviors. One such critical behavior is cell migration into and within the injury site. Understanding and controlling the migratory capability of 3D-cultured cells is therefore pivotal for developing better transplantation techniques. ECM remodelling can influence numerous cellular functions, including cell migration and matrix metalloproteinases (MMPs) are important enzymes for ECM modulation. Here, we discuss the idea of modulating MMPs to control cell migration in 3D culture systems, which can improve the therapeutic potential of cells transplanted in 3D.
引用
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页数:11
相关论文
共 126 条
[1]   Schwann Cell Migration is Integrin-Dependent and Inhibited by Astrocyte-Produced Aggrecan [J].
Afshari, Fardad T. ;
Kwok, Jessica C. ;
White, Linda ;
Fawcett, James W. .
GLIA, 2010, 58 (07) :857-869
[2]   Nerve guide material made from fibronectin:: Assessment of in vitro properties [J].
Ahmed, Z ;
Underwood, S ;
Brown, RA .
TISSUE ENGINEERING, 2003, 9 (02) :219-231
[3]  
Ahmed Z, 1999, CELL MOTIL CYTOSKEL, V42, P331
[4]  
Ahmed Z, 2000, CELL MOTIL CYTOSKEL, V46, P6
[5]   Comparative Analysis of 3D Bladder Tumor Spheroids Obtained by Forced Floating and Hanging Drop Methods for Drug Screening [J].
Amaral, Robson L. F. ;
Miranda, Mariza ;
Marcato, Priscyla D. ;
Swiech, Kamilla .
FRONTIERS IN PHYSIOLOGY, 2017, 8
[6]   Co-transplantation of olfactory ensheathing glia and mesenchymal stromal cells does not have synergistic effects after spinal cord injury in the rat [J].
Amemori, Takashi ;
Jendelova, Pavla ;
Ruzickova, Katerina ;
Arboleda, David ;
Sykova, Eva .
CYTOTHERAPY, 2010, 12 (02) :212-225
[7]  
Aono S, 2006, ADV PHARMACOL, V53, P323, DOI 10.1016/S1054-3589(05)53015-1
[8]   Realistic modeling of tumor cells [J].
van Golen, Kenneth L. .
ONCOTARGET, 2017, 8 (16) :25833-25834
[9]   Cell transplantation therapy for spinal cord injury [J].
Assinck, Peggy ;
Duncan, Greg J. ;
Hilton, Brett J. ;
Plemel, Jason R. ;
Tetzlaff, Wolfram .
NATURE NEUROSCIENCE, 2017, 20 (05) :637-647
[10]   Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures [J].
Balgude, AP ;
Yu, X ;
Szymanski, A ;
Bellamkonda, RV .
BIOMATERIALS, 2001, 22 (10) :1077-1084