Short-wavelength two-photon excitation fluorescence microscopy of tryptophan with a photonic crystal fiber based light source

被引：43

作者：

Palero, JA ^{[1
]}

Boer, VO

Vijverberg, JC

Gerritsen, HC

Sterenborg, HJCM

机构：

[1] Univ Utrecht, NL-3584 CC Utrecht, Netherlands

[2] Erasmus Univ, Med Ctr, Ctr Opt Diagnost & Therapy, NL-3000 CA Rotterdam, Netherlands

来源：

OPTICS EXPRESS | 2005年 / 13卷 / 14期

关键词：

D O I：

10.1364/OPEX.13.005363

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report on a novel and simple light source for short-wavelength two-photon excitation fluorescence microscopy based on the visible nonsolitonic radiation from a photonic crystal fiber. We demonstrate tunability of the light source by varying the wavelength and intensity of the Ti:Sapphire excitation light source. The visible nonsolitonic radiation is used as an excitation light source for two-photon fluorescence microscopy of tryptophan powder. (C) 2005 Optical Society of America.

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页码：5363 / 5368

页数：6

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