Short-wavelength two-photon excitation fluorescence microscopy of tryptophan with a photonic crystal fiber based light source

被引：43
|
作者：
Palero, JA [1 ]
Boer, VO
Vijverberg, JC
Gerritsen, HC
Sterenborg, HJCM
机构：
[1] Univ Utrecht, NL-3584 CC Utrecht, Netherlands
[2] Erasmus Univ, Med Ctr, Ctr Opt Diagnost & Therapy, NL-3000 CA Rotterdam, Netherlands
来源：
OPTICS EXPRESS | 2005年 / 13卷 / 14期
关键词：
D O I：
10.1364/OPEX.13.005363
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We report on a novel and simple light source for short-wavelength two-photon excitation fluorescence microscopy based on the visible nonsolitonic radiation from a photonic crystal fiber. We demonstrate tunability of the light source by varying the wavelength and intensity of the Ti:Sapphire excitation light source. The visible nonsolitonic radiation is used as an excitation light source for two-photon fluorescence microscopy of tryptophan powder. (C) 2005 Optical Society of America.
引用
收藏
页码：5363 / 5368
页数：6
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