Effects of different extraction temperatures on the physicochemical properties of bioactive polysaccharides from Grifola frondosa

被引：88
作者：
Su, Chun-Han [1 ]
Lai, Min-Nan [2 ]
Ng, Lean-Teik [1 ]
机构：
[1] Natl Taiwan Univ, Dept Agr Chem, 1,Sec 4,Roosevelt Rd, Taipei, Taiwan
[2] Kang Jian Biotech Co Ltd, Nantou, Taiwan
来源：
FOOD CHEMISTRY | 2017年 / 220卷
关键词：
Grifola frondosa; Mushrooms; Water soluble polysaccharides; Physicochemical properties; PHELLINUS LINTEUS POLYSACCHARIDES; ANTITUMOR-ACTIVITY; GANODERMA-LUCIDUM; IN-VITRO; MAITAKE; FRACTION; MACROPHAGES; EXPRESSION; MUSHROOMS; CELLS;
D O I：
10.1016/j.foodchem.2016.09.181
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
This study examined the effects of different extraction temperatures (70 degrees C, 100 degrees C and 121 degrees C) on the physicochemical properties of water soluble polysaccharides (WSP; GF70, GF100 and GF121, respectively) from Grifola frondosa (GF) fruiting bodies, and evaluating their effects on nitric oxide (NO) production in lipopolysaccharide-stimulated RAW264.7 macrophages. Results showed that GF121 had the highest yield. GF70, GF100 and GF121 contained a similar monosaccharide composition and the predominant monosaccharide was glucose. These polysaccharides contained two major macromolecular populations; the high molecular weight population showed a clear trend of reduced molecular weight with increasing extraction temperature. GF121 contained the highest amount of (1 -> 3, 1 -> 6)-beta-D-glucans, while the degree of branching in all samples was similar. GF WSP possessed NO inhibitory activity, and the strongest was GF121. This study concludes that WSP are good sources of food ingredients, and high temperature extraction could improve the quantity and quality of GF WSP. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：400 / 405
页数：6
相关论文
共 31 条
[1]   THERMAL-DENATURATION OF (1-]3)-BETA-D-GLUCANS IN NEUTRAL AQUEOUS-SOLUTION ABOVE 130-DEGREES - EFFECT ON PHYSICOCHEMICAL PROPERTIES [J].
ADACHI, Y ;
OHNO, N ;
YADOMAE, T ;
SUZUKI, Y ;
OHSAWA, M ;
OIKAWA, S .
CARBOHYDRATE RESEARCH, 1990, 198 (01) :111-122
[2]   CHANGE OF BIOLOGICAL-ACTIVITIES OF (1-]3)-BETA-D-GLUCAN FROM GRIFOLA-FRONDOSA UPON MOLECULAR-WEIGHT REDUCTION BY HEAT-TREATMENT [J].
ADACHI, Y ;
OHNO, N ;
OHSAWA, M ;
OIKAWA, S ;
YADOMAE, T .
CHEMICAL & PHARMACEUTICAL BULLETIN, 1990, 38 (02) :477-481
[3]   Correlation of branching structure of mushroom β-glucan with its physiological activities [J].
Bae, In Young ;
Kim, Hye Won ;
Yoo, Hyun Jae ;
Kim, Eun Suh ;
Lee, Suyong ;
Park, Dong Yun ;
Lee, Hyeon Gyu .
FOOD RESEARCH INTERNATIONAL, 2013, 51 (01) :195-200
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   Extraction and characterization of hemicelluloses from flax shives by different methods [J].
Buranov, Anvar U. ;
Mazza, G. .
CARBOHYDRATE POLYMERS, 2010, 79 (01) :17-25
[6]   A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].
DUBOIS, M ;
GILLES, K ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
NATURE, 1951, 168 (4265) :167-167
[7]   Mushroom immunomodulators: unique molecules with unlimited applications [J].
El Enshasy, Hesham A. ;
Hatti-Kaul, Rajni .
TRENDS IN BIOTECHNOLOGY, 2013, 31 (12) :668-677
[8]  
HISHIDA I, 1988, CHEM PHARM BULL, V36, P1819
[9]   Relationship between solubility of grifolan, a fungal 1,3-β-D-glucan, and production of tumor necrosis factor by macrophages in vitro [J].
Ishibashi, K ;
Miura, NN ;
Adachi, Y ;
Ohno, N ;
Yadomae, T .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2001, 65 (09) :1993-2000
[10]   Maitake D-fraction enhances antitumor effects and reduces immunosuppression by mitomycin-C in tumor-bearing mice [J].
Kodama, N ;
Murata, Y ;
Asakawa, A ;
Inui, A ;
Hayashi, M ;
Sakai, N ;
Nanba, H .
NUTRITION, 2005, 21 (05) :624-629
1234