Asymmetric synthesis of (R)-1,3-butanediol from 4-hydroxy-2-butanone by a newly isolated strain Candida krusei ZJB-09162

被引:23
作者
Zheng, Ren-Chao [1 ,2 ]
Ge, Zheng [1 ,2 ]
Qiu, Zhao-Kuan [1 ,2 ]
Wang, Yuan-Shan [1 ,2 ]
Zheng, Yu-Guo [1 ,2 ]
机构
[1] Zhejiang Univ Technol, Minist Educ, Engn Res Ctr Bioconvers & Biopurificat, Hangzhou 310014, Zhejiang, Peoples R China
[2] Zhejiang Univ Technol, Inst Bioengn, Hangzhou 310014, Zhejiang, Peoples R China
关键词
(R)-1,3-Butanediol; Candida krusei ZJB-09162; Carbonyl reductase; Asymmetric reduction; NADH-dependent; CARBONYL REDUCTASE; PURIFICATION; 1,3-BUTANEDIOL; BIOCATALYSTS; SPECIFICITY; ENZYME; CELLS;
D O I
10.1007/s00253-012-3942-2
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Biocatalytic asymmetric preparation of (R)-1,3-butanediol has been attracting much attention in pharmaceuticals industry. A new ideal strain, ZJB-09162, which exhibited high reduction activity and excellent (R)-stereospecificity towards 4-hydroxy-2-butanone, has been successfully isolated from soil samples. Based on morphology, physiological tests (API 20 C AUX), and 5.8S-ITS sequence, the isolate was identified as Candida krusei. Kinetic characterization demonstrated that carbonyl reductase from C. krusei ZJB-09162 preferred NADH to NADPH as cofactor, indicating it might be a new carbonyl reductase. (R)-1,3-Butanediol was produced in 19.8 g/L, 96.6% conversion, and 99.0% ee at optimal pH 8.5, 35 A degrees C with a 2:1 molar ratio of glucose to 4H2B. In order to achieve higher product titer, the substrate loading was optimized in fixed catalysts and fixed substrate/catalysts ratio mode. The bioreduction of 4-hydroxy-2-butanone at a concentration of 45.0 g/L gave (R)-1,3-butanediol in 38.7 g/L and 83.9% conversion. Therefore, C. krusei ZJB-09162 was, for the first time, proven to be a promising biocatalyst for enzymatic preparation of (R)-1,3-butanediol.
引用
收藏
页码:969 / 976
页数:8
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